Carbon Monoxide Inhalation Protects Rat Brain, Liver And Kidney Tissues From The Injury After Ischemia Reperfusion On Double Hind Limbs

Objective: To investigate the protective effection on the ischemia reperfusion (IR) on the double hind limbs injured brain, liver and kidney tissues through inhaled carbon monoxide (CO).Methods: 100 SD rat were divided randomly into 4 groups, 25 SD rat in each group were divided further into 6h, 24h, 48h, 72h, 10d groups according to the time when obtained specimens. Of 4 groups, IR group: rats underwent 4-hour bilateral femoral artery occlusion of hind limb to produce ischemia followed by reperfusion; IR and inhaled CO group(RC): rats underwent 4-hour ischemia of hind limb followed by reperfusion, with inhaling CO after 2-hour ischemia for 4 hours; sham operation group(S): rats underwent sham operation which just expose bilateral femoral artery without IR; S and inhaled CO group(SC): rats underwent sham operation, and inhaled CO 2-hour later for 4 hours. 6h and 10d groups within which samples were taken after reperfusion for 6 hours or 10 days; the former followed by HE stain, and the later followed by flow cytometry. Within 24, 48, 72h groups, the blood samples were taken after reperfusion for 24, 48, 72 hours to detect the liver and renal function.Results: 1:There is no difference between S and SC groups. 2 : Compared with that of group SC: In IR, pathological change of hippocampus, liver and kidney tissue in 6h group were significant; In 24, 48, 72 groups, plasma GPT were obviously increased, and were depressed by turns; in 24h group, plasma k+, Cr, BUN were increased; in 48h group, BUN were increased; in 10d group, the percentage of apoptosis, the FI of proteinum bax were increased; the FI of proteinum bcl-2 of kidney tissue were increased. 3 :Compared with that of group IR: In RC, pathological change in 6h group were slight; plasma GPT in 24, 48, 72h groups were decreased; in 24h group, plasma k+, Cr, BUN were decreased; in 48h group, plasma BUN were decreased; in 10d group, the percentage of apoptosis, the FI of proteinum bax of hippocampus, liver and kidney tissues were depressed,the FI of proteinum bcl-2 of these tissues were increased.Conclusions: The ischemia reperfusion on the double hind limbs induced the injury of hippocampus, liver and kidney tissue. Inhaled CO can attenuate injury of these tissues.