Study On Preventive And Therapeutic Function Of Compound White Peony Root Oral Liquids In Treating Experimental Radiation-induced Esophagitis

Objective: The preventive and therapeutic function of compound white peony root oral liquids (cWPROL) were studied. The mechanism of therapeutic effect to rats'experimental acute radiation-induced esophagitis by cWPROL were analyzed with studying on restorative function for immunodeficiency, content of the hydroxyl radical (OH) and NO, activity of CAT and GSH-PX, and the change of EGF, TGFβ1, iNOS and SP expression in rats'esophagus tissue, NO level in blood serum.Methods:1 128 Wistar rats were divided into eight groups. Group 1, normal group, was without any radiated and treatment; Group 2, radiated group 1, were sacrificed on the seventh day after irradiation by 43Gy 60Co-γray; Group 3, radiated group 2, were sacrificed on the fifteenth day after irradiation; Group 4 were infused with preventive (normal dose cWPROL) at the same time of radiation; Group 5 were infused with preventive (high dose cWPROL) at the same time of radiation; Group 6 were infused with cWPROL (normal dose) from the seventh day after irradiation; Group 7 were infused with cWPROL (high dose) from the seventh day after radiation; Group 8 were treated with Western medicine from the seventh day after radiation.2 The preventive and curable function of cWPROL and Western medicine were observed from the aspects of pathological change, ultrastructure change, average weight of food and volume of water englobed by rat.3 On every experimental point the absolute number and percentage of T lymphocyte subsets were analyzed by flow cytometry. The changes of the leukocyte number and differential count were detected by haemocyte counting instrument. IgG and C3 in rats'serum were analyzed by immunological turbidimetry.4 Another 96 Wistar rats were divided into eight groups and treated same to method 1. On every experimental finished point, the serum and full-length esophagus of rats were kept for examination. The content of hydroxyl radical (OH) and NO, and activity of CAT and GSH-PX in rats'esophagus tissues were determined. The content of NO in rats'serum was also analyzed.5 On every experimental point, expression of EGF, TGFβ1, iNOS and SP were detected by immunohistochemistry and collagen fibrils distribution in esophagus tissue were observed by Masson stain.Results:1 Both of cWPROL and Western medicine can treat the inflammation of esophagus induced by radiation. Ultrastructure group of rat treated with preventive (normal dose) by cWPROL recovered approximately. The weight of food and volume of water increased in the group infused with trad. Ch. medicine compared with the radiated group 2, especially in the group treated with preventive (high dose). The weight of food englobed by the rats which were treated with Western medicine decreased compared with the radiated group 2.2 Radiation can brought decreasing of leukocyte count, lymphocyte differential count, absolute count and absolute T lymphocyte subsets in the blood and the content of IgG and C3 with radiation esophagitis in experimental rats. cWPROL could increase the lymphocyte percentage compared with the radiated group 2. The preventive (high dose) could increase lymphocyte count while Western medicine decreased rats'leukocyte count, lymphocyte percentage and absolute count (P <0.05). Absolute T lymphocyte subsets in rats'peripheral blood was increased in the group infused with cWPROL (high dose) (P <0.001). Compared with radiated rats (group 2), absolute T lymphocyte subsets decreased significantly(P<0.001), and the content of complement C3 increased in the rats infused with cWPROL (high dose) (P <0.05), but decreased in the rats infused with Western medicine (P <0.01).3 The content of hydroxyl radical (OH), NO and in Wistar rats'esophagus tissue increased significantly compared with the normal rat on the 14th day radiated by 43Gy 60Co-γ( P <0.05), and the activity of CAT and GSH-PX increased significantly (P <0.01). However, the content of hydroxyl radical (OH●), NO and the activity of CAT, GSH-PX in the esophagus tissue of rat treated with cWPROL decreased significantly compared with the rat radiated (group 2) (P <0.05), the rat treated with cWPROL (high dose) from the seventh day after radiation (P <0.01). The content of NO and the activity of CAT, GSH-PX in the esophagus tissue were reduced in the rats treated with Western medicine (P <0.05) compared with the rats radiated group 2, especially the content of NO decreased significantly (P <0.01). The content of NO in blood serum was no significantly different between all of the groups (P >0.05).4 On day 14^th after radiated by 43 Gy 60Co-γ?ray, the expression of EGF and TGFβ1 in the esophagitis tissue of rats increased compared with the normal rats. Expression of EGF and TGFβ1 in the rats infused with preventative cWPROL were higher than the radiated group2 (P<0.05) and higher significantly than the normal group (P <0.01). Expression of EGF and TGFβ1 in the rats treated with cWPROL from day 7th after irradiation had no significant difference compared with the radiated rat (group 2) (P >0.05), while higher than the normal group (P <0.05). Expression of TGFβ1 in the rats treated by the normal dose of cWPROL from day 7th after irradiation was no significantly difference from the normal group (P >0.05), while by the high dose of cWPROL, expression of TGFβ1 expression was higher than the radiated group 2 (P <0.05). By treatment with Western medicine, expression of EGF in esophagitis tissue of rats was higher than the radiated rats (P <0.05) and significant higher than the normal rats (P <0.01), TGFβ1 expression was higher than the normal rats (P <0.05), while no significant difference than the radiated rats (group 2) (P >0.05).5 iNOS expression in propria lamina increased significantly in the radiated group 1 and 2. Compared with the radiated group 2, iNOS expression in propria lamina decreased in the rat treated with preventive and curative medicine significantly (P <0.01).6 On day 7th and 14th radiated by 43 Gy 60Co-γ?ray, substance P expression increased significantly compared in normal rats (P <0.01). After treated with preventative cWPROL, substance P decreased significantly compared with the radianted group 2(P <0.01)and recovered into normal level (P >0.05). Substance P expression in the rat treated with cWPROL from the day 7th after irradiation was lower than radiated rats (group 2) (P <0.01), but not recovered into normal level (P <0.05). Treatment with Western medicine, substance P expression in the rats'esophagus tissue was a little lower than radiated rats (group 2) (P <0.05) and significantly different than normal rats (P <0.01).Conclusion:1 cWPROL were beneficial from aspect of prevention and therapy on radiation-induced esophagitis. 2 cWPROL had a role of restoring the cytoimmunity and immunity damaged in rats with radiation esophagitis by radiation.3 cWPROL can reduce the content of hydroxyl radical (OH), NO and activity of GSH-PX and CAT in esophagus tissue of rat radiated with 60Co-γ?ray, which maybe is the mechanism for the treatment of inflammation of esophagus induced by radiation.4 cWPROL can shorten the time course of intention by increasing expression of EGF and TGFβ1 then declined when the pathological scathe induced by radiation recovered.5 cWPROL can inhibit NO excessive synthesis in esophagus tissue of rat with radiation induced esophagitis by decreasing iNOS expression and then educed the function of promoting the intention of esophagus.6 cWPROL may lighten the symptom of pain induced by radiation esophagitis and promote the esophageal intention by shortening the time from the high level of substance P to normal and inhibiting the excessive expression of substance P.