Studies On Image Processing And Analysis Method For Red Blood Cell Deformability Measurement System Based On Silicon Micro-channel

The deformability of Red blood cell (RBC) is one of important blood rheology indexes. Therefore, the study of RBC deformability has played an important role in preventing and curing some certain diseases refer to RBC morphological features. Above all, this dissertation shows a kind of cell deformability analysis system developed by ourselves which is based on MEMS and microfluidic technology. Compared with normal rheological detection technology, this method is beneficial on many ways, such as multi-information, flexible and controllable structure, easy to handle and low price.The emphasis of my dissertation is image acquisition and analysis for the RBC deformability testing system based on silicon microchannel. On the one hand, collecting the voltage signals from the photodiodes for further analysis, including protract the flux- filtrate volume curve of RBC suspension and calculate the two parameters in the RBC deformability theoretical model automatically by computer. On the other hand, we use the high speed imaging system to capture images when the RBC suspension get across the microchannels. For the motive images of RBCs, I calculate the velocity and acceleration when the RBC suspension get across the microchannels in order to analyze the entrance effect, and then compare the RBC deformability. For the still image, I presented a improved method of contour extraction accurately for RBC image, and then analyze the RBC deformability by the way of calculating the perimeter and landmeasure of the RBC. The program compiling tool of my dissertation are Visual C++ and Matlab.In the present dissertation, a new semi-automatic data/image processing and analysis method for RBC deformability testing has been developed. And this approach is an economical, objective, precise and can provide valuable reference for the auxiliary diagnosis of relative diseases, as well as other applications in the fields of biomedicine and biomedical engineering. The study also makes the foundation in the field of hemorheology for the further development of this new method.