The Relationship Of HPV16 Infection And The Expression Of P33～(ING1b), HTERT In Cervical Cancer Of Uygur And Han People Female In Sinkiang

Object: To investigate the correlationship among the infection of HPV16, the expression of p33^ING1b and hTERT, their clinical significance as well as the pathogenesy of cervical carcinomatous affectivity of Uygur female in Sinkiang.Methods: Collected the paraffin embeded tissue and clinicopathological data of 90 cases cervical cancer, 69 cases cervical intra-epithelial neoplasia and 21 cases normal cervix in People's hospital of Xingjiang Uygur Autonomous Region from January of 2000 to June of 2005. Based on histopathological observation, HPV16 DNA were detected by using Polymerase chain reaction; at same time, p33^ING1b, hTERT of cervical tissue were detected by immunohistochemical method.Results: (1) The proportion of HPV16 DNA of 96 cases cervical cancer of Uygur female was 74.0% (37/50), which was higher than control group 0 (0/12), the defference was significant (x 2u=26.537,pu=0.000). The proportion of HPV16 DNA of 84 cases cervical cancer of Han people female was 65.0% (26/40), which was higher than control group 11.1% (1/9), the defference was significant (x 2h=13.504,ph=0.004). (2) The positive proportion of p33^ING1b protein in SCC group of Uygur was 36.0% (18/50), which was lower than control group 91.7% (11/12), the defference was significant (x 2u =38.943,pu =0.000). The positive proportion of p33^ING1b protein in well-differentiated SC group, moderately-differentiated SC group, poorly- differentiated SC group respectively was 77.7%, 22.7%, 31.6%. Compared with CINIII group, the moderately-differentiated SC group (x 2u =10.140,pu =0.004) and poorly-differentiated SC group (x 2u =8.304,pu =0.005) of Uygur were all lower, the defference were all significant. The positive proportion of p33^ING1b protein in SCC group of Han people was 55.0% (22/40), which was lower than control group 100% (9/9), the defference was significant (x 2h =32.672,ph =0.000). Compared with CINIII group, poorly-differentiated SC group of Han people was lower, the defference was significant (x 2h =13.482,ph =0.003). (3) The positive proportion of hTERT protein in SCC group of Uygur was 96.0% (47/50), which was higher than control group 50.0% (6/12), the defference was significant (x 2u =48.199,pu =0.000). The defference between the positive proportion of hTERT protein and tissular differentiation in cervical cancer of Uygur was not significant (pu >0.05) . The positive proportion of hTERT protein in SCC group of Han people was 90.0% (36/40), which was higher than control group 22.2% (2/9), the defference was significant (x 2h =27.776,ph =0.001). The defference between the positive proportion of hTERT protein and tissular differentiation in cervical cancer of Han people was not significant, too (ph >0.05). (4) Among cervical tissue of two nations, the infection rate of HPV16 and the expression of p33^ING1b protein were negative correlation, the defference was significant (r u =-0.294,p u =0.004;r h =-0.216,p h =0.049). But the infection rate of HPV16 and the expression of hTERT protein were positive correlation in cervical tissue of two nations, the defference was significant (r u =0.286,pu =0.005;r h =0.291,ph =0.007). In addition, the expression of p33^ING1b protein and the expression of hTERT protein were negative correlation, the defference was significant (r u =-0.361,pu =0.000;r h =-0.285,ph =0.009). (5) The positive proportion of p33^ING1b protein in SCC group of Uygur was 36.0% (18/50), which was lower than Han people 55.0% (22/40), the defference was significant (x 2 =6.557,p=0.038). But the defference of the inffection rate of HPV16 and the expression of hTERT protein between the Uygur and the Han people were not significant (p>0.05).Conclusion: (1) The infection rate of HPV16 in invasive carcinoma of cervix increased to explain that the infection of HPV16 was correlated to cervical cancer. (2) The expressed rate of p33^ING1b protein in invasive carcinoma was lower, and its expression of Uygur was lower than Han people, explained that ING1 gene and p33^ING1b protein may inhibit the development of tumor, moreover this effective existed defference among defferent nations. (3) High expression of hTERT protein in CIN and invasive cancer, which explained that telomerase activation might appearance in morning of cervical carcinomatous development, and it was impormant to the development of tumor. (4) It was negative correlation between the infection of HPV16 and the expression of p33^ING1b protein, which explained that p33^ING1b could inhibit the cancerogenesis of HPV16 by some pathway; It was positive correlation between the infection of HPV16 and the expression of hTERT protein, which explained that they could cooperate each other in the tumorigenesis; It was negative correlation between the expression of p33^ING1b protein and hTERT protein, we conjectured that p33^ING1b protein and p53 protein might cooperate to inhibit the cancerogenesis of hTERT, which was on account of the p33^ING1b protein and the hTERT were all intimate correlated to p53 protein.