| In recent years, Because of the new drugs' searching and exploitation having a broad clinical practice prospect, people pay more and more attention on Chinese medicine polysaccharide. Chinese medicine animal model is an important subject of modernized study of Chinese medicine theory. The standard animal model of Chinese medicine is significant to study the cause of disease, pathogenesis and develop treatment and prevention.This paper firstly introduces the Chinese medicine polysaccharide's development and application in recent years, and studies its extraction, separation, purification, structural analytical methods, biological activity identification and clinical application and summarizes the evolution of the Chinese medicine animal model.The major purpose of this study is going to set up a new animal model of Chinese medicine from predecessors. Here, we choose angelica polysaccharide to study the effect on type 2 diabetic animal model (NIDDM), then we could built a new Chinese medicine animal model on the anti-insulin action of NIDDM.First, some polysaccharides fractions were isolated and purified form dry angelica and analyzed the characters of each fraction. The type 2diabetes models of C57 BL/6J mice were formed by injecting middle dosage of streptozotocin (STZ). Then they were fed with angelica polysaccharide. Type 2diabetes mice will be cured by angelica polysaccharide. Measure and result as follows:1. Primary study on isolate and characters of angelica polysaccharide. Hot water extracting was used to isolate total polysaccharide (AP0) from dry angelica. Treated AP0 with Cetyltrimethyl Ammonium Bromide (CTAB), three fractions were isolated, named AP1, AP2 and AP3, respectively. The content of polysaccharide is determined by phenol-sulphuric acid method, results showed that the polysaccharide contents were 89.1%, 81.5%, 76.3% and 78.6%, respectively. Spectral analysis suggested that four polysaccharides have common spectral characters of polysaccharides and the glucoside bond were a-D-glucopyranose. Studies on the four polysaccharides by atomic force microscopy indicated that molecule of angelica polysaccharides were muff-branched structure, single molecular chain as well as side branches and polymer chain moleculars were twisted with each other.2. Primary study on structure of derivate angelica polysaccharide.Preparation of Carboxymethylated and sulfated polysaccharides were by solvent method and chlorosulfonic acid pyridine method, respectively. Fourier transform infrared spectroscopy indicated that both of two polysaccharides have typical characteristic spectrum of angelica polysaccharide. Carboxymethylated polysaccharide has c, arbonyl characteristic absorption peak at 1609cm1, 1420 cm1, in which was a sharp peak of methylene. The two peaks showed that the polysaccharide was Carboxymethylated. There are apparent absorption peaks at 823 cm-1 and 1254 cm-1, in which were absorption peaks of C-O-S and sulfated group of sulfated polysaccharide. Their existent revealed that the polysaccharide was sulfated.3. Effect of angelica polysaccharide on type 2diabetic animal model.Thirty C57 BL/6J mice were divided into two groups: twenty mice are model group which injected 1% STZ 50mg/kg each, others (group A,10) were injected buffer solution equally. Three days later, fasting plasma glucose was tested in tail blood. When the NIDDM model was established, the model mice were randomly divided into two groups: NIDDM model group(group B) and others (group C) were treated by angelica polysaccharide for two weeks. Each of diabetes treated mice was fed with 5% angelica polysaccharide solution 1.0g/kg everyday. Model group and black group were gave 0.3ml normal saline. Later, fasting plasma glucose was tested in eyeball blood. The result showed that angelica polysaccharide can reduce the blood glucose of NIDDM, but the hypoglycemic mechanism is not clear.
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