| Objective To investigate the expression of delta opioid receptor during kidney ischemia reperfusion injury and make a basic experiment for clinical organ transplantation.Methods Rat models of kidney ischemia reperfusion injury(IRI) were established,all rats's right kidney were ligated. Twenty healthy male SD rats were randomly divived into four groups(each containing five rats):(1)Sham operation group(control),rats's left kidney pedicle were only separated,not blocked;(2)IRI group,rats experienced 1 hr ischemia and then followed with different reperfusion intervals(0.5,6,24hr).The level of serum blood urea nitrogen and creatinine were analysised with BECKMAN COULTER SYNCHRONL×20 instrument.The level of DORmRNA in kidney were analysised by the real-time fluorescence quantitative polymerase chain reaction.Result After 60min ischemia and 30min,6hr and 24hr reperfusion respectively,the serum BUN and Cr was (8.460±0.07234,114.9±8.467),( 14.34±1.225, 180.5±9.739),( 44.45±1.969, 423.3±19.75) respectively, which was obviously higher than the sham group, which was (2.940±0.1386,72.18±5.976),all p<0.01.Namely the renal function of all IRI groups was significantly lower than that of the sham group.The level of DOR mRNA of the IRI groups made for 30min, 6hr, 24hr was respectively 74.29±0.7724, 68.47±18.95,1.325±0.6704,which was obviously lower than that of the sham group which was 354.2±55.99(all p<0.05 as compared with the sham group).Conclusion there is the expression of DOR mRNA in the rat kidney,and the level of DORmRNA is obviously decresed in IRI,which suggests that DOR might have a important role when kidney was injured by ischemia reperfusion. |
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