Effects Of GSH On Plasma Level Of TNF-α,IL-6 And Organ Ultrastrusture In Sepsis Rats.

Objective To observe effects of GSH on plasma level of TNF-α,IL-6 and pulmonary,liver,kidney ultrastrusture in sepsis rats,and then ,to investigate the mechanism of the organ ultrastrusture injury in sepsis rats and the protection effects of GSH.. Method The sepsis rats were established by cecal ligation and puncture(CLP).112 male SD rats were randomized into four groups,e.g. sham operated group(sham),CLP model group(CLP) , GSH treated group(GSH) and levofloxacin treated group(LEV).Each group was subdivided into 4 groups,e.g. 3 hours group(T3),6 hours group(T6),12 hours group(T12) and 24 hours group(T24). The changes of plasma TNF-αand IL-6 were determined at different time points.At the same time,the ultrastructure injury of lung,liver and kidney were observed by electron microscope. Results In CLP group,the plasma level of TNF-αin 6 hours group increased more obviously than the GSH treated group,s (P<0.01).The plasma level of IL-6 in CLP group rised after three hours of CLP.In GSH treated group ,the plasma level of IL-6 after .three hours of CLP was lower than CLP group (p<0.05). In CLP group ,the ultrastrusture injury of lung,liver and kidney was obviously,especially in the mitochondria of each organ.In GSH treated group,the ultrastructure of each organ changed lightly. Conclusion The mechanism of the organ ultrastructure injury in sepsis rats is associated with the role of TNF-αand IL-6.The sepsis rats treated by GSH were presented effective distinctively.