Objective To purify placental immunoregulatory factor furtherly,surveying and evaluating its liveness.To investigate the treatment effects of placental immunoregulatory factor on the experimental endometriosis in rats.Methods Placental immunoregulatory factor(PF),which was extracted from the placenta of healthy women,was purified throught SepHadexG-25 gel filtration chromatography.The constituents purified were detected with E.rosettes on the human full blood.A2780 cells were treated by placental immunoregulatory factor and the constituents purified,the MTT assay was used to analyse the growth of A2780 cells.The rats with experimental endometriosis were randomly divided into 5 groups,control,model,and three treatment groups with placenta immunoregulatory factor.After eight weeks of treatment with placental immunoregulatory factor,diameters of implants were measured and the morpHological changes observed,serum interleukin -2(IL-2)was determined by EL1SA,acetic acid nonspecific esterase(ANAE)activity in peripHeral blood was detected with histochemical mothod,the proliferation response of splenocyte was evaluated by ATP bioluminescence assay and peritoneal macropHages were evaluated by neatral-red method,the expressions of interleukin-6(IL-6)mRNA of spleen tissues were assayed by reverse transcription polymerase chain reaction (RT-PCR). Results Four constituents were obtained form placental immunoregulatory factor and the fourth constituent was proved to have E.rosettes on the human full blood.The growths of two A2780 cell lines were obviously inhibited by placental immunoregulatory factor and the fourth constituent purified.After treatment,the volumes of the ectopic implants of each treatment group were significantly smaller.Placental immunoregulatory factor could increase level of interleukin-2(IL-2)and acetic acid nonspecific esterase(ANAE)activity. Placental immunoregulatory factor could increase the macrophages phagocytosis. Placental immunoregulatory factor of high dose and middle dose could obviously increase the lymphocyte proliferation response.The expressions of interleukin-6(IL-6)mRNA of spleen tissues were decreased.Conclusions The fourth constituent,which had E.rosettes on the human full blood,was purified from placental immunoregulatory factor and it could inhibite A2780 cell line.Placental immunoregulatory factor can increase cellular immune function of the rat endometriosis.The right dosage of placental immunoregulatory factor could play treatment role in growth and conglutinafion of rat EMS model. |