The Preparation Of Sodium Citrate Injection For Catheter Locking

Objective: To develop a sodium citrate for catheter locking which has the advantages of anticoagulant and antimicrobial properties. Methods: The imitative setting of indwelling catheter in vitro was established by which the prescription and technics were screened. High performance liquid chromatography was taken to determine the content of citrate in the prescription. Its stability was investigated through accelerated test and long-term test. The anticoagulant effect and the antibacterial activity of the injection were studied in vitro. Its security was investigated by hemolytic test in vitro and the acute toxicity in mice in vivo. Results: The raw material was made in China and the technics of preparation was conventionally taken. The quality of sodium citrate injection for catheter locking could be controlled and the prescription in this research could be remained for at least 72 hours in the retained catheter. The period of validity of the prescription was more than 18 months. Its anticoagulation was better than that of heparin. Sodium citrate injection for catheter locking had favorable antimicrobial efficacy against pathogen accounting for infection of indwelling catheter. The haemolysis and coagulation on red blood cells of rabbits in vitro of the sodium citrate injection were not significant. The median lethal dose (LD50) of sodium citrate injection for catheter locking was 0.305g/kg in mice. Conclusions: The sodium citrate injection for catheter locking prepared in this research, which has the advantages of anticoagulant and antimicrobial properties with simple procedure of preparation and steady characteristics, is a promising substitute for heparin sodium injection.