The Effect Of Antidepressant To The Neurogenesis In The Rat Hippocampus Of Depression Model

Objective To study the mechanism of different antidepressant about the neurogenesis in the rat hippocampus of depression model.Method The depression model of rat was produced by separation and chronic unpredicted mild stress.The behavior was measured by open-field and fliud consumption test.Then intragastric administration with fluoxetine,tianeptine and normal sodium. Administration for 4 weeks, the expression of SYN protein ,NSE protein and GFAP protein were measured by immunohistochemisty to observe the neurogenesis.Result This animal model of depression seems indeed meet the three validity criteria necessary to validate an animal model of depression,chronic stress can make changes of morphology and microstructure and cell loss in hippocampus neuron. Compared with the normal sodium control group, the expression level of SYN,NSE and GFAP potein in hippocampus was increased in fluoxetine group and tianeptine group.And the expression level of NSE was increased in tianeptine group compared with the fluoxetine group. The number of neuron and astrocyte in hippocampus was decreased in stress group compared with normal group. After administration for 4 weeks, the number of neuron and astrocyte in hippocampus was increased in fluoxetine group and tianeptine group as compared with normal sodium group.Conclusion The rat model of depression has been established successfully.Chronic fluoxetine or tianeptine treatment significantly increases the neurogenesis in the rat hippocampus of depression model.