Studies On Hypoglycemic Activity And Influence To The Microorganism's Growth Of Polysaccharide From Nature Spirulina Platensis

Polysaccharide from spirulina platensis (PSP) have hypoglycemic effect. Spirulina cultivated in Erdos Inner Mongolia is first crashed, dispored of fat, extracted then precipitated protein by sevage method finally separated by vacuum freezing drying. The raw polysaccharide is further purified by column chromatography on DEAE—52 and SephardexG—200. The refined polysaccharide is achieved. The polysaccharide fraction is examined by electrophoresis paper and gel chromatography, the results showed the fraction is a homogenous substance. After the gas chromatography analysis it is indicated that PSP contain xylose fructose glucose and the other monosaccharide derivative. In addition ,SephardexG—200 gel chromatography showed the average molecular weight of the polysaccharide is 35800D.With the dose of 100mg/kg , 200mg/kg by ig administration to normal for 10 days,it is found that that PSP had no significant hypoglycemic effect on normal mice. In addition,with the same dose by ig administration, PSP could decrease the hyperglycemia that is induced by intraperitoneal adrenaline and by ig glucose in mice. Such a result showed that PSP may antagonize the glycogenolysis which is stimulated by adrenaline in liver and inhibit the absorption of glucose in intestinal in the mice. The above results suggested that PSP posses certain hypoglycemic effect and deserve to further studying.To study the effect of the polysaccharide of Spirulina platensis on promoting the growth of Bifidobacteria.adolescentis and Lactobacillius in vitro. It appears that polysaccharide of Spirulina platensis significantly promoted the growth of them(P<0.1).In addition the antimicrobial characteristics of PSP is tested.