Relationship Between The Levels Of Serum Adiponectin And Hs-C Reactive Protein In Insulin Resistant Rats

Type 2 diabetes mellitus (T2DM) is an innate immunity and chronic inflammationdisease. In susceptible individual of T2DM, as the effect of environmental factor suchas aging and overnutrition,the innate immune system was activated ,which causeInsulin Resistance(IR),and lead to the development of metablic syndrome and T2DM.Adiponectin, exclusively derived from adipose tissue, could increase insulin sensitivity,the inflammation process in IR can effect the synthesis and secretion of APN, cause thedecrease of plasma APN. C reactive protein(CRP)is the most susceptive index of acutephase protein in inflammation.IR can increase synthesis of CRP,this study is try toapproach the relationship between APN and CRP in IR rat, providing a theoreticalevidence to the multitarget treatment of IR.ObjectiveTo observe the relationship between serum adiponectin(APN) and high sensitiveC reactive protein(CRP) in insulin resistant rats.MethodsInsulin resistant rat model was induced by high-fat diet feeding for 10 weeks,then assessed by euglycemic-hyperinsulinemia clamp technique (EHCT). The serumlipid assay was performed with enzymatic measure, the free fatty acid (FFA) was testedwith colorimetry, the serum adiponectin,insulin and hs-CRP was determined byenzyme linked immunosorbent assay (ELISA).Results 1. High-fat diet in which fat affording 71% caloric, induced insulin resistant ratmodel. The average glucose infusion rate (GIR) during 60～120min of EHCT in HFgroup decreased significantly compared with NF group. The fasted seruminsulin(FINS), tfiglyceride(TG) and FFA in HF group increased significantly comparedwith NF group. The serum adiponectin level and insulin sensitivity index (ISI) in HFgroup decreased significantly compared with NF group.the serum hs-CRP level in HFgroup increased significantly compared with NF group.2.The correlation between serum APN and hs-CRP level in insulin resistant rat isnegative.ConclusionThe serum APN level decreased in insulin resistance rat,while hs-CRPincrease,the correlation between the two is negative.