Location Of Serotonin Receptor 1A In The Spiral Ganglion With LsAB Technique

Objective The serotonin is a widespread neurotransmitter in the centralnervous system (CNS), It plays an important role in modifying excitatory effectof nerves which is accomplished with the special serotonin receptor subtypesfrom the nerves. At present, at least seven different families of serotonin receptorshave been identified and each family of these receptors has two or more subtypes.For example the serotonin receptor 1 family consists of serotonin receptor 1A,serotonin receptor 1B, serotonin receptor 1D, serotonin receptor 1E, and serotoninreceptor 1F. Those functions are accomplished with the special serotonin receptorsubtypes that use different second messengers, which are coupled to distinct ionchannels. Some researches are presented that the serotonin can inhibit excitationof nerves when they combine with the serotonin receptors 1A which are locatedin the nerves. We know that the serotonin receptor 1A survives in the spiralganglion which was identified with RT-PCR technique, but we do not know itsspecific localization in the spiral ganglion. So in the present study, we locate theexpression of serotonin receptor 1A in the spiral ganglion with LsAB technique.Methods In the mouse(born between three to six days) cochlear frozen slides,serotonin receptor 1A in the spiral ganglion was located with LsAB technique(labeled streptavidin-biotin technique). Results Positive signals were seen inthe membrane of spiral ganglion As a negative control with no serotonin receptor1A antibody, no signal was found in the membrane of spiral ganglion. ConclusionThe serotonin receptor 1A has been found in the membrane of spiral ganglion. Itindicates that serotonin as a neurotransmitter maybe play an important role ininhibiting the auditory transmission of the spiral ganglion by the way that it combines with the serotonin receptor 1A which is located in the membrane of thespiral ganglion when it is stimulated by the hair cells to be excitatory.