| Purpose: To investigate the expression of the IL-1β,IL-6 and the TNF-a in the prostate gland liquid of chronic non-bacterial prostatitis (CP/CPPS),discuss the relations between occurrence of CP/CPPS patients and partial out-balance of cytokines ; Observe the influence of cytokines IL-1β,IL-6 and the TNF-a in EPS of CP/CPPS patients by specific COX-2 inhibitor celecoxib; To evaluate the effect of clinical pain improvement of CPPS patients through celecoxib and observe side effect of celecoxib.Methods: All patients should be urinate examination before and after massage of prostate and germ culture. Meanwhile we calculate the average of WBC in 3-5 high power visual field through micrography of EPS. All patients were classified into two groups according to PPMT and diagnosis standard of prostatitis of NIH: the IIIA group include 34 cases, the IIIB group include 30 cases ,and control group include 10 health male. All cases were detected the level of IL-1β﹑IL-6 and TNF-ain EPS through RIA. Again we detected the level of IL-1β﹑IL-6 and TNF-ain EPS of CP/CPPS group who take celecoxib for two weeks with 0.2g qd. All IIIB cases should be NIH-CPSI and classified into two groups :one group take celecoxib 0.2g qd, another group take celecoxib 0.2g bid .All cases should be NIH-CPSI after two weeks. The result were statistical analyzed with t-test.Result: Each group the level of the IL-1βin EPS:IIIA group is 2.17±s 0.56, the IIIB group didn't detect, the control group is 1.02±s 0.29. Each group the level of the IL-6in EPS:IIIA group is 93.91±s 7.74, IIIB group is 58.77±s 7.56, the control group is 27.60±s 4.53. Each group the level of the TNF-ain EPS:IIIA group is 3.89±s 2.50, IIIB group is 3.35±s 0.48,the control group is 2.01±s 0.34.The level of IL-1βin EPS after take celecoxib for two weeks: IIIA group is 1.98±s 0.27, the IIIB group didn't detect. The level of IL-6 in EPS after take celecoxib for two weeks: IIIA group is 127.31±s 4.32, IIIB group is 88.40±s 5.88. The level of TNF-αin EPS after take celecoxib for two weeks: IIIA group is 3.44±s 0.52, IIIB group is 3.20±s 0.43.The level of TNF-α,IL-1β,IL-6 in EPS of IIIA patients is higher than the control group after and before take celecoxib(P﹤0.05).The level of TNF-αand IL-1βin EPS was decreased after take celecoxib ,but there were no significant difference with before take medicine﹙P﹥0.05). The level of IL-6 in EPS was rised after take celecoxib,and show significant difference with before take medicine﹙P﹤0.05). The level of TNF-αand IL-6 in EPS of IIIB patients is higher than the control group after and before take celecoxib(P﹤0.05). The level of TNF-αin EPS was decreased after take celecoxib ,but there were no significant difference with before take medicine﹙P﹥0.05).The level of IL-6 in EPS was rised after take celecoxib, and show significant difference with before take medicine﹙P﹤0.05).The level of IL-Iβin EPS was not detected before and after take medicine. The IIIB group pain score is 12.433±s 1.87 and NIH-CPSI score is 25.500±s 3.41.A group (take celecoxib 0.2 g, qd) pain score is 8.533±s 1.33 and NIH-CPSI score is 19.833±s 2.34 after two weeks. B group(take celecoxib 0.2 g, Bid) pain score is 9.833±s 1.71and NIH-CPSI score is 21.291±s 2.84 after two weeks .There show significant difference between before and after treatment in pain score and NIH-CPSI score two weeks later in A and B group. But there were no statistical significance between A group and B group(P﹥0.05). There were no side effect of drug obviously .Conclusion: The cytokine TNF-aand IL-6 increase in EPS of the III type CP/CPPS patients, and maybe play the important role in morbidity and pathogenesis of CP/CPPS. The cytokine IL-1βincrease in EPS of the IIIA type CP/CPPS patients ,and did not detected in EPS of IIIB type CP/CPPS patients before and after take medicine. We conclude it maybe participate morbidity of IIIA type CP/CPPS and it is difficult to predict that IL-1βinfluence morbidity of IIIB type CP/CPPS in detect condition at present. Celecoxib can increase the lever of IL-6 in EPS of TNF-a obviously ,and decrease the lever of TNF-ain EPS of III type CP/CPPS patients and the lever of IL-1βin EPS of IIIA type CP/CPPS patients partially.Low-dose of celecoxib relieved pain of CP/CPPS IIIB,while increased dose did not show any advantage. No obvious side effect was observed. |
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