Expression Of Cyclooxygenase-2 And Inducible Nitric Oxide Synthase In Laryngeal Squamous Cell Carcinoma And Their Correlation With The Angiogenesis

Purpose To study the expreesion of cyclooxygenase-2(COX-2) and inducible nitric oxide synthase(iNOS) in laryngeal squamous cell carcinoma(LSCC) through immunohistochemistry,and to understand the correlation between the expression of COX-2 with iNOS in LSCC,and the relationship between the angiogenesis of LSCC with the expression of COX-2 and iNOS by detecting microvessel density(MVD).Methods 48 samples of paraffin was embed laryngeal squamous cell carcinoma were collected for the present study.These samples were cut off from surgical operations and stored in pathologic section of the people's hospital of Hunan province from 2001 to 2006. 20 samples normal squamous epidermis near cancer and 20 samples laryngeal polypi were selected.The patients' age, sex, differentiation, lymph node transfer and TNM stage were recorded.All samples were recuted to diagnose. The pathological specimen was cut in the thickness of 4um. SP immunohistochemistry staining for COX-2,iNOS and MVD were made.The result of immunohistochemistry were determined by the intensity of staining and the number of positive cells.The expressions of COX-2,iNOS in LSCC,normal squamous epidermis near cancer and laryngeal polypi were analysed.The relationgship between the expression of COX-2,iNOS with the clinical pathology characteristics,and the expression of COX-2 and iNOS were done.The MVD in LSCC,normal squamous epidermis near cancer and laryngeal polypi,and the relationship between the expression of COX-2, iNOS with MVD,and the relationship between the dyeing intensity of COX-2,iNOS in LSCC with MVD were analysed.Also the relationship between the type of pathology typing with MVD was determined.Result1 The expression of COX-2 in LSCC was up-regulated which had significantly different (P<0.05) from that of both LSCC and normal squamous epidermis near cancer or laryngeal polypi. There was not significant correlation between the expression of COX-2 with the age of patient,the type of pathology ,and lymph node transfer.However there was a significant correlation between the expression of COX-2 with the tumor TNM stage.2 The expression of iNOS in LSCC was upregulated, which was significantly different (P<0.05) from that of both LSCC and normal squamous epidermis near cancer or laryngeal polypi. There was not significant correlation between the expression of iNOS with the age of patient and the tumor TNM stage, but there was significant correlation between the expression of iNOS with the type of pathology or lymph node transfer.3 The expression of COX-2 and iNOS had positive correlation (r=0.490;P<0.01).There was a cooperation between the expressions of COX-2 with iNOS in LSCC.4 There was a significant difference(P<0.05) between the MVD expressions in LSCC with in normal squamous epidermis near cancer or laryngeal polypi.The MVD was much higher in COX-2 positive LSCC or iNOS positive LSCC than in COX-2 negative LSCC or iNOS negative LSCC(P<0.05). The MVD was significantly higher in both COX-2 and iNOS positive LSCC than any other groups(P<0.05).There was a significant correlation between COX-2 with MVD (r=0.54,P<0.01).There was also a significant correlation between iNOS with MVD (r=0.30,P<0.05).There was not a significant correlation between the intensity of the expression of COX-2 or iNOS(P>0.05) with MVD.But there was a significant correlation between the pathology typing of LSCC with MVD.Conclusion1 Either COX-2 or iNOS may accelerate the genesis or progress of LSCC.2 There may be a cooperation between COX-2 and iNOS in the genesis and progress of LSCC.3 The high density of MVD in LSCC relates to COX-2 and iNOS.The genesis or progress of LSCC by accelerating the genesis of new vessel may be affected by COX-2 and iNOS.