Study On Variation Of Quantities Of T Lymphocyte Subsets And NK Cells In Peripheral Blood Of Patients With Epithelia Ovarian Carcinoma

Objective:To investigate the significance of detection of T lymphocyte subsets and NK cells in peripheral blood of patients with epithelia ovarian carcinoma,and if T lymphocyte subsets and NK cells more excellent than CA125 and CA199 in diagnosis of epithelia ovarian carcinoma.Methods:Detection variation of quantities of T lymphocyte subsets and NK cells in peripheral blood of patients with epithelia ovarian carcinoma by flow cytometry. Data obtained from the benign epithelial ovarian tumor and the health people were usedas control.Results:1.Comparing quantities of T lymphocyte subsets and NK cells in peripheral blood of patients with epithelia ovarian carcinoma and control groups. Lowers CD4+,CD4/CD8 ratio and NK cells were detected in peripheral blood samples from carcinoma group than that from the control group(P﹤0.05),and CD8+ were reverse.These changes was related to the pathological stages.2. Comparing quantities of CA125 and CA199 in peripheral blood of patients with epithelia ovarian carcinoma and control groups.Highers CA125 and CA199 were detected in peripheralblood samples from carcinoma group than that from the control group (P﹤0.05),especially inⅢstage andⅣstage of carcinoma group(P﹤0.01).These changes was related to the pathological stages. 3. Comparing positive rates of T lymphocyte subsets , NK cells,CA125,CA199 and B-Ultrasonic in peripheral blood of patients with epithelia ovarian carcinoma in different stages.The highest positive rate is CD16+CD56+,81.25% in diagnosis in early Stage of epithelia ovarian carcinoma, then CD4/CD8 ratio(62.50 % ) , CA125+CA199(50%),B-Ultrasonic(50%),CA125(43.75%)and CA199(12.50%).While in diagnosis in early stage of epithelia ovarian carcinoma, the highest positive rate is 88.89%, obtained by CD16+CD56+, CD4/CD8 and B-Ultrasonic. That is higher than CA125+CA199(77.78 % ),CA125(77.78 % )and CA199(16.67 % ). Comparing positive rates, only CD4/CD8 and CA199, CA199 and CD16+CD56+ have significant differences.4. Comparing positive rates of T lymphocyte subsets , NK cells,CA125, and CA199 in peripheral blood of patients with epithelia ovarian carcinoma in different pathologic types.The positive rate of CA125(80%) is more than CA199(12%) in ovarian serous adenocarcinoma, and there is significant difference(P﹤0.05).While the positive rate of CA199(55.56%) is more than CA125(20.22%) in ovarian mucoid adenocarcinoma, but there is no significant difference(P>0.05). The positive rate of CD4/CD8 and CD16+CD56+ are 84.00% and 92.00% in ovarian serous adenocarcinoma, while they are 55.56% and 66.67% in ovarian mucoid adenocarcinoma,and all of these have no significant difference(P>0.05).5. Comparing detection of T lymphocyte subsets , NK cells,CA125, and CA199 in peripheral blood of patients with epithelia ovarian carcinoma.The highest sensitivity in diagnosis are combination detection of CA125+CA199+CD4/CD8+NK and B-Ultrasonic+CA125+CA199+CD4/CD8+NK,which is 94.12%. The highest specificity in diagnosis is combination detection of CA125+CA199+ B-Ultrasonic, 72 % . The highest correct index in diagnosis is combination detection of B-US+CA125+CA199+CD4/CD8+NK,54.12%.Conclusions:1. The quantities of CD4/CD8 ratio and CD16+CD56+ are lower in early stage of ovarian epithelial carcinoma, and they are decreased with the developing state of carcinoma.2. The sensitivity and correct index in diagnosis of CD4/CD8 ratio and CD16+CD56+ are more excellent than CA125 and CA199. The positive rate in diagnosis in early stage of ovarian epithelial carcinoma of CD4/CD8 ratio and CD16+CD56+ may be more excellent than CA125 and CA199.3. Combination detection of CD4/CD8, CD16+CD56+ , CA125 and CA199 could elevate the sensitivity in diagnosis, but there is no significant changes in specificity and correct index.