Experimental Study In Vitro About The Apoptosis Induction Effects Of Photodynamic Therapy On Ovarian Cancer Cell Line Skov3 And Cervical Cancer Cell Line Hela

Objective:Photodynamic therapy is one of the new methods of tumor treatment,which are developed in recent years. The method is to kill the tumor cellsthrough photosensitizing reactions. The main advantages of the methodare the high precision selection for the tumor cells and the tissuespecificity. That is, when the tumor cells are killed by the therapy, thehuman normal tissue cells are little damaged. Although photodynamictherapy is applied in many fields in recent years, there are still fewreports in gynachologic field. The purpose of present experimental studyis to oberserve anti-proliferation and apoptosis induction effects ofphotodynamic therapy on cervical cancer line Hela and ovarian cancercell line Skov3 cultured in vitro.Methods:Cervical cancer cell line Hela and ovarian cancer cell line SKOV3treated by photosensitizer 5-ALA at different doses were radiated withlaser, After 48 hours, morphological characteristics were observed bylight microscopy and electronic microscopy, The effects of photodynamictherapy on the cancer cell growth and apoptosis in vitro were measuredby MTT assay and FCM. One-way ANOVA was adopted as statisticanaylyses method.Results: The cytomorphosis and shrinkage, nucleus condensation andanachromasia, dense chromatin lining along cell membrane wereobserved under inverted microscope in cell line receiving photodynamictherapy. Through transmission electron microscope(TEM), we found thatnucleus chromatin gathering along membrane of cell nuclear andapoptotic body formed. Through MTT essay, the cell inhibition rateswere 12.9%-90.7% and 3.9%-70% in Skov3 cell and HeLa cell treatedwith 5-ALA of different concentrations (0.1, 0.5, 1.0, 2.5mM) andreceiving laser of different doses (0.1, 0.5, 2.5, 12.5J/m~2) respectively. Asthe statistical analysis for the experimental results shows, theconcentration of the photosensitizer and the dose of laser had effects onthe optical density(OD) of ovarian cancer cell line Skov3 and cervicalcancer cell line Hela(p＜0.01). It is also shown, that the interactionbetween the photo sensitizer and laser was very strong(p＜0.01).Theoptical density of the two cell lines decreased with the increases of photosensitizer concentration and laser dose (P＜0.01); therefore, apparentdependency of the results on the photo sensitizer concentration and laserdose had been shown. The effects of photodynamic therapy for differenttype of cells were also different. The tendency of decrease of lightabsorption value was not apparent when the photo sensitizerconcentration＞1.0mM and dose of laser＞2.5/cm2 for cervical cancercell line Hela. The maximum inhibition rates were 68.2% and 60.5% in Skov3 cell and HeLa cell respectively, examined by flowcytometry(FCM). Diversity is also observed.Conclusion:Photodynamic therapy inhibits the growth of ovarian cancer cell lineSkov3 and cervical cancer cell line Hela in vitro.