Apoptosis And The Expression Of Releated Proteins Induced By Ar-He-based Targeted Cryosurgery In Lung Adenocarcinoma

Objective:To investigate the possible mechanism of killing lung carcinoma cells except direct necrosis induced by Ar-He-based targeted cryosurgery in vitro and in vivo. Methods :The experimental models-the LA795 cells cultured on the cover glass and mouse with adenocarcinoma, were established, and they were subjected to cryo-treatment via argon-helium-based cryocare system. Then cell apoptosis in situ was examined with TUNEL staining.The expression of bcl-2 protein and bax protein was detected by immunohistochemichal procedures. Results: In the center of the cryolesion ,cell death occurred maily by means of necrosis. But in the periphery of the cryolesion, cell death occurred maily by means of apopotosis determined by morphological analysis and TUNEL staining,and two high levels of apopotosis were observed.The expression of bax protein increased significantly,meanwhile the expression of bcl-2 protein had no obviously change.Conclusion: The Ar-He-based targeted cryosurgery can induce cell apoptosis in the periphery of the cryolesion by upregulation of bax expression but not bcl-2 expression. And chemotherapy or radiotherapy can be combined to improve the cryosurgery efficiency by enhacing apoptosis.