Triptorelin Reverses Cisplatin Resistance In Human Ovarian Carcinoma Cell Line (OVCAR-3) Via MAPK-ERK1/2 Signal Pathway

Objective: to determine the effect and mechanism of triptorelin on cisplatin-resistant OVCAR-3 ovarian carcinoma cell line in vitro. Methods: Cisplatin-resistant cell subline OVCAR3-DDP was induced. After treated respectively with cisplatin, triporelin, and combination (cisplatin and triptorelin) in different concentration, the proliferation, cycle, apoptosis and ERK1/2 activation of OVCAR3-DDP cells were detected by MTT assay, flow cytometry and Western blotting, respectively. Results: .The resistant index of OVCAR3–DDP cells was 3.87, and the reversal reaction (RR) induced by combination was 2.23; ERK1/2 activation in OVCAR3–DDP cells was up-regulated by cisplatin, but was down-regulated by triptorelin and combination. Conclusions: Triptorelin can increase the cytotoxic effects of cisplatin on OVCAR3-DDP cells in vitro by modulating the activity of the MAPK-ERK1/2 signaling pathway.