The Effect Of Transformation Growth Factor β1 (TGF-β1) In Antiapoptosis Of Degenerated Intervertebral Disc Cell In Rats

The effect of transformation growth factorβ1 (TGF-β1) in antiapoptosis of degenerated intervertebral disc cell in ratsObjectiveThe intervertebral disc draws back changes disease (disc degeneration disease, DDD) is one kind of disease incidence rate very high disease. At present thought the intervertebral disc draws back changes origins from the nucleus pulposus, the cell is weak perishes is excessively a primary cause which the nucleus pulposus cell draws back changes. But the growth factor retreats is an important cause which the cell perishes weakly. Some research indicated the function which TGF-β1 regarding draws back through the injection which changes the intervertebral disc cell has prevents it to draw back changes. This research through observes the big mouse intervertebral disc to draw back changes the model after the injection transformation growth factorβ1 (TGF-β1), the cell perishes weakly the morphological change and the cell perish weakly rate change, from gene level discussion TGF-β1 in anti- counter intervertebral disc degeneration function.Materials and MethodsThis experiment selects the male Wistar big mouse (China Medical University experimental animal center to provide), heavy 300g, altogether 48. Divides into two parts stochastically: A part does not make any processing, altogether 12, are called the blank control group; Another group makes the intervertebral disc to draw back changes the model, altogether 30. Before makes the mold latter 2 weeks, has not carried on further experiments, these two parts of distinctions select 3 big mouse executions stochastically. The second part of surplus 27 big mouse stochastically divides into three groups again: The injection TGF-β1 group (below called the TGF group), the injection physiological saline control group (below calls salt water group), the non-treatment group. Each group of altogether 3. This three group of 27 big mouse same blank control group surplus 9 big mice, each group stochastically divides into 2 week, 4 week, 8 Wednesday time spot. Each time selects n=3. Executes after separately, each group of big mouse same sequence makes the mold mark place to take the new organization block. Perishes weakly with the haematoxylin Iraq red dyeing observation intervertebral disc the cell shape, dyes the observation color reaction with TUNEL, and counts the masculine cell number to express the cell perishes weakly the index.Results1. Pair of ordinary light microscope paraffin section HE dyeing carries on the observation discovery, after makes the mold 2 weeks, makes the mold in the experimental animal intervertebral disc to appear massively perishes weakly the cell, confirmed makes the mold to be successful. But after the injection TGF-β1 2 weeks, the TGF group's intervertebral disc perishes the cell to start weakly to reduce, explained the cell already started to repair. But with it correspondence, the salt water group, the non-treatment group perishes the cell not to see the reduction weakly. After 4 week, 8 weeks, the TGF group perishes the cell further to reduce weakly, but the salt water group and the non-treatment group perishes the cell still not to see the reduction weakly. 2. Uses the home position to perish the reagent box to carry on the examination weakly to the paraffin wax embedding specimen, calculates perishes weakly the index (apoptosis index, AI). Makes the mold latter 2 weeks, makes mold train AI same blank control group AI to compare the difference obviously (P＜0.01), proved makes the mold to be successful. The injection TGF-β1 after each time spot, the TGF group with between the salt water group, the non-treatment group compares the difference obviously (P＜0.01), has statistics significance; The salt water group compares the not obvious difference with the non-treatment group between (P＞0.05), non-statistics significance; Between TGF group each time compares the difference to be remarkable, (P＜0.01), has statistics significance; But the TGF group, the salt water group, between the non-treatment group same blank control group compares the difference obviously also (P＜0.01), has statistics significance.Conclusion1. This research through observes the big mouse intervertebral disc to draw back changes the model after the injection transformation growth factorβ1 (TGF-β1), the cell perishes weakly the morphological change and the cell perish weakly rate the change, discovered TGF-β1 has the anti-intervertebral disc to draw back changes the function which the cell perishes weakly. 2. TGF-β1 cannot completely cause the intervertebral disc cell which draws back changes to perish weakly obtains the correction.