Effect Of Aluminum Exposure On LTP And PKC γ Protein And MRNA Express In Hippocampus Of Pups During Pregnancy And Lactation Matrix

PrefaceAluminium is one of the most redundant elements in the crust of the earth.Athough it is not highly noxious, but too much of it in our body can cause toxicity.There are many investigations and researches show that aluminium is most likely causeneuron impairment and decrease mentality and recognization. It is confirmed by animalexperiments that exposure of aluminium can cause rats dementia, which is not onlyappearances behavioral disturbance but also it's morph changes are most like AD.Hippocampus is the key brain region of study and memory, Long-TermPotentiation is synaptic transmission's continuity enhancing of the NMDA receptorsdepended. It is received study model of study and memory's function of brain at thesynapse level. It's induction and maintenance is depended on the multiple fore-synapticand post-synaptic. Consequently, studying the effect of aluminium on Long-TermPotentiation and approaching it's possible mechanisms can elucidate the mechanisms ofaluminium on study and memory.The research indicated that the pregnant and lactational stage is the importantstage for the filial brain growth and development. Effect of aluminum exposure at thisstage on the filial brain is a topic worthy of being studied thoroughly. Therefore, wemade the parent rats exposed to aluminum and took their pups as the subjectsinvestigated to study the effect of aluminum exposure on the formation, maintain ofLTP, and tried to elucidate the mechanism of aluminum impairing to LTP. Material and Methods1. Animal and Treatment30 female Wistar rats aged 4 weeks and weighing about 180g were randomlydivided into three groups of 10 animals. Animals from the control group receiveddistilled water ad libitum as unique source of liquid. Animals from the experimentalgroup received a aluminium chloride solution (0.2% and 0.4%) ad libitum, as uniqueliquid source, female rats were administered aluminum chloride from the first day ofpregnancy to the ending of lactation. All pups changed to drink distilled water andbelonged to the group of its mother. When weighing about 200 g, pups were killed forexperiment.2. Methods(1) Ethology detectionEthology detection was detected by jumping stand experiment.(2) LTP recordThe technique of extracellular electrophysiological was used torecord the effect ofaluminium on long-term potentiation (LTP) in hippocampal CA1 area.(3) Aluminium concentration analysisAtomic absorption spectrophotomery(AAS) was used to detect the content ofAluminium in blood and brain.(4) The changes on expression of PKCγWesten-blot method(5) The changes on expression of PKCγmRNART-PCR methodResults1. Aluminium concentration in blood and brainThe blood aluminium concentration in the rats of aluminium exposed groups weremuch higher than those of control groups. And there were significant changes in lowand high aluminium exposed groups. The same changes were there in brain aluminium concentration except the significant changes in low and high aluminium exposedgroups.2. Ethology detectionThe learning and memory were significant decreased in in the rats of aluminiumexposed groups. The latency period was significant longer than the control group,andthe error times was significant increased than the control group.3. LTP recordThe population spike(PS) in hippocampal CA1 was depressed in the rats ofAluminium exposed groups. And the higher dose the aluminium exposed the mucherdepressed.4. The change on PKCγprotein and mRNA expressionThe aluminium exposed groups were much lower than those of control groups onon PKCγprotein and mRNA expression. And following the blood aluminiumconcentration increasing, The aluminium exposed groups take on degraded tendency.The significant changes was found in low and high aluminium exposed groups.Conclusions1. Chronic aluminum exposure during pregnant and lactational stage can damagethe function of learning and memory of pups.2. Chronic aluminum exposure during pregnant and lactational stage has aninhibitory effect on the formation of LTP in CA1 area of pups.3 Chronic aluminum exposure during pregnant and lactational stage can degradethe expresion of PKCγlevel and mRNA of pups. provid the theory for frommolecular level elucidate Chronic aluminum exposure can damage the function oflearning and memory...