Effect Of Cyclosporin A Eye Drop On Keratoconjunctivitis Sicca And Its Mechanism

objective: In this study, the effect of cyclosporin A (CsA) eye drop on keratoconjunctivitis sicca (KCS) and its mechanism were studied.Methods: The keratoconjunctivitis sicca (KCS) models were established by injecting Pertussis vaccine, complete freund's adjuvant (CFA) and antigen of conjunctiva from isotype mice. Then the keratoconjunctivitis sicca (KCS) models were treaded with cyclosporin A eye drop. Changes in breaking-up time (BUT), lacrimal secretion in 30 min and diversion in 24h were measured. The percentage of beaker cells, the lymphocytic infiltration in conjunctiva were observed. The expression levels of Aquaporin-3 (AQP3) in conjunctiva epithelial cells, beaker cells and accessory lacrimal gland were immunohistochemically detected.Results: The results showed that there were significant differences in breaking-up time (BUT), the percentage of beaker cells, lacrimal secretion in 30 min, the lymphocytic infiltration and the expression of Aquaporin-3 (AQP3) among the different groups.but 1% cyclosporin A (CsA) eye drop had injury to eye surface.Conclusion: It was concluded that cyclosporin A (CsA) eye drop exerts marked therapeutic effect on keratoconjunctivitis sicca (KCS) by inhibiting T lymph cells, increasing the goblet cells and Aquaporin-3 (AQP3) expression in conjunctiva.