Experimental Study On The Extract Of The Leaves Of Juglans Mandshurica Maxim Against Irradiation Damage

Ionizing radiation has been used to treat many malignancies since the turnof the century. Modern radiotherapeutic techniques have led to increased curerates and improved local regional control, but the potentially severe anddebilitating adverse effects of radiation therapy have not been fullyeliminated.Managing radiation-induced toxicities often focuses on treating thesymptoms as they manifest. However, preventing this complication is clearlymore desirable. The use of chemical radioprotectors represents an obviousstrategy to improve the therapeutic index in radiotherapy. Among the currentlyaviailable chemical radioprotectors, Lipoic acid derivatives represent the mostimportant group of radioprotective compounds, however, their toxicityassociated with hypotension, nausea, and emesis has limited their developmentfor application to medicine. Although the radioprotective drugs acting viadifferent mechanisim was used at the same time can markedly improve thedegree of protection and keep toxicity to acceptable levels in small rodents, touse such treatments in large mammals have been less successful. It is a focus tofind out more effective radioprotective agents to enhance the radiotherapytherapeutic benefit for cancer patients without increasing their risk of seriousadverse effects. The purpose of this paper was to study the protective effects ofthe Alcohol Extract of the Leaves of Juglans mandshurica Maxim (AELJ) on X-ray irradiated mice, and hoping to find a new Radiation Protection drugs.The main results of the study are as follows:1. Analysis of the composition of AELJ: Chemical and TLC methods wereused to detective the components of AELJ, the experimental results show that the main constituents of AELJ are phenolic and tannin compounds, flavonoids,saponins, organic acids, and anthraquinones.2. The acute toxicity test of AELJ in mice: The maximum dose test wasused to determin the acute toxicity of AELJ. The results showed that, the largestvolume of AELJ is more than 18g/kg, and the toxicity of AELJ is lower.3. Antioxidation hemolysis test on the RBC of AELJ in vitro: To determinethe protective effects of AELJ on the hemolysis of red blood cells induced bythemselves and H2O2. The results show that AELJ can inhibit the oxidationhemolysis of red blood cells, and has dose-effect relationship obviously.4. To evaluate the protective effects of AELJ on mice with irradiationdamage induced by X-ray, the mice were randomly divided into six groupsincluding normal control group, irradiating control group, low AELJ plusirradiating group, highAELJ plus irradiating group, low AELJ drugs only groupand highAELJ drugs only group.5. The effect of AELJ on hematopoietic in irradiated mice in vivo.Compared with the Irradiating control group, AELJ could delay the decline ofthe quantity of white blood cells effectively in circulation due to ionizingirradiation; AELJ could increase the content of serum iron of mice; AELJ couldprevent the decreasing of the nucleated cells in bone marrow.6. The effect of AELJ on immune function in irradiated mice. Comparedwith the Irradiating control group, AELJ could impove the index of thymus andspleen in ionizing irradiation rats; AELJ could enhance the ability oflymphocyte transformation.7. The protective effect of AELJ on oxidative injury of the liver tissue inradiated mice.Compared with the Irradiating control group, AELJ could decrease the level of malondiadehyde (MDA); AELJ could increase the activityof superoxide dismutase (SOD).In words, the raw material of AELJ could obtain easily, and the toxicity ofAELJ is lower; AELJ has evidently protective effects on acute injury caused byionization radiation.These results have not been reported previously.