The Effects Of BRD On Expresstions Of EGF And EGFR In Rat With Gastric Ulcer

Peptic ulcer (peptic ulcer, PU) is a gastrointestinal mucosa and submucosa muscle necrosis pathological injury, the need for healing epithelial tissue and connective tissue reconstruction. H2 receptor antagonists selectively binding on the membrane wall of the H2 receptor, the cell wall of cAMP generated, reduce gastric acid secretion. H2 receptor antagonist not only to histamine-stimulated acid secretion is inhibited, but also partially inhibited gastrin and acetylcholine-stimulated acid secretion, and promote mucosal ulcer healing. EGF is a 53 amino acid composition of the single-chain polypeptide,Mainly come from the submandibular gland, duodenum Brunner gland, renal tubular cells, pancreas, thyroid and liver, etc,In the gastric mucosa can also detect low levels of EGF protein. Through its specific EGF receptor (EGFR) combined to play in maintaining its integrity and mucosal injury and repair in the process of important biological role.Objective:Through the observation of the new H2-receptor antagonist-the effects of butylamine roxatidine derivate(BRD) on expresstions of EGF and EGFR in rat with gastric ulcer,discussion is BRD acetate for the promotion of peptic ulcer healing mechanism.Methods:48 from Wistar rats were randomly divided into six groups, each with eight, namely: (1) control group, to appropriate saline gavage (2) ulcer model control group, to appropriate saline gavage (3) to (5) butylamine roxatidine derivate(BRD) high for the small acetate, , the low-dose group (28 mg / kg, 14mg/kg, 7mg/kg) (6) drug Ranitidine positive control group (27 mg / kg). (2) to (6) rats using acetic acid burning Preparation gastric ulcer model, not the normal control group rats surgery.Rats after 15 days of continuous intragastric administration in the last administration after fasting for 24 hours, free drinking water, the first 16 days will be broken cervical rats were sacrificed, drawing. HE staining of butylamine roxatidine derivate(BRD) for small acetate rat mucosa of the gastric ulcer healing the impact of immunohistochemical staining of gastric EGF and EGFR expression.Results:1. HE staining of the rats ulcer formation and healing: Normal control group: the rat gastric antral wall structural ntegrity, glandular mucosa neat, and there was no obvious inflammatory cell infiltration.Model group: Gastric telangiectasia, congestive, focal bleeding obvious that a large number of interstitial infiltration of inflammatory cells, and some regional mucosal necrosis, exfoliated, local glands damaged.Treatment group: ranitidine, butylamine roxatidine derivate(BRD) small acetate for high-dose and the dose of mucosal ulceration were observed varying degrees of healing ulcer lesions have varying degrees of the surface epithelium new coverage, inflammatory cells decreased significantly, we can see that the nascent gland hyperplasia coverage ulcers and fibrous connective tissue, obviously at the bottom of ulcer formation of granulation tissue, vascular mild gastric expansion, congestive.2. Renewable rat gastric mucosal thickness, the muscularis mucosa defects of the results of comparison:Renewable thickness of the gastric mucosa statistical analysis shows that for butylamine roxatidine derivate(BRD) acetate in small doses and high dose group, ranitidine group and the untreated group model has obvious significant difference, p <0.01 .Comparing the muscularis mucosa defects of the statistical analysis showed that butylamine roxatidine derivate(BRD) small acetate for the high dose group, ranitidine group and group therapy model has obvious significant difference, p <0.01.3.Experimental gastric ulcer rat mucosa EGF and EGFR immunohistochemical staining:Immunohistochemical staining showed that the normal control group EGF and EGFR expression was weakly positive for small butylamine roxatidine derivate(BRD) high dose group and the positive drug Ranitidine group EGF and EGFR expression was significantly increased. butylamine roxatidine derivate(BRD) for the small-dose group increased expression of EGF and EGFR not obvious. These data suggest that n-butylamine Jiluosha acetate for the treatment of small ulcer in the process, for the dose-dependent expression of EGF and EGFR, EGF and EGFR may be mediated by di-n-butylamine Jiluosha for small acetate promote ulcer healing role.Conclusion:The successful establishment of the rat gastric ulcer model. butylamine roxatidine derivate(BRD) study found that for a small increase in acetate ulcer mucosal lesions and the surrounding renewable thickness, width decreased muscularis mucosa defects, and promote epithelial tissue regeneration capabilities, and improved weary scar repair, butylamine roxatidine derivate(BRD) study found that for a small increase in acetate ulcer mucosal lesions and the surrounding renewable thickness, width decreased muscularis mucosa defects, and promote epithelial tissue regeneration capabilities, and improved weary scar repair, Granulation tissue, there by stimulating the formation of microvascular, inhibit secretion of parietal cells, and stimulate the ulcer edge epithelial cell proliferation, differentiation, migration and increased mucosal blood flow, and enhance the mucosal regeneration glands function, and promote ulcer healing.