Expression And Significance Of SULT1E1 Gene In Endometrial Adencarcinoma Tissues

ObjectiveEndometrial carcinoma is one of the three most frequent malignancy in female genital tract, which is the most common pelvic genital cancer in women now. It's about 7%of all female carcinoma. Studies on etiology of endometrial carcinoma haven't draw the conclusion yet. The most scholars thought the endometrial carcinoma arising, continues with the estrogen to stimulate the endometrium for a long time and to lack the progestin to antagonism close correlation, each kind of reason estrogen too much, is the main reason which the endometrial carcinoma arises. Sulfotransferase( SULTs) superfamily including phenols (SULT1, PST) and hydroxyl steroids (SULT2, HSST) transfer of the two sub-group. SULT1E1 is SULT1 one of the main members of the family,.which is the strongest enzyme of the 10 know isoforms catalyzing estrogen at present ,which catalyzes the transfer of a sulfonate radical (SO₃^-) to the 3-hydroxyl group of estrogens using 3'-phosphoadenosine-5'-phosphosulfate as a donor for the (SO3") group. As important disintoxicating enzyme class, SULT1E1 catalyzes the sulfation of estrone and estradiol with extremely high efficiency, detoxification by which a relatively hydrophobic xenobiotic is biotransformed into a more water-soluble sulfuric ester, reducing the level of estrogen exposion in the circulation and the target organization. Studies demonstrated that dereased SULT1E1 expression may resut in estrogen -dependent tumor developing . Hower the relaton of estrogen sulfotransferase and endometrial carcinoma has not been examined.The article investigates the protein expression and the mRNA expression of SULT1E1 and research relationship between the occurrence and development of endometrial adenocarcinoma, the study will provide significant assistance to studies on etiology of endometrial adenocacinoma. Methods1. PatientsIn this study, the samples were chosen from benign and malignant endometrium 72 cases who suffered from hysterectomy at Department of Gynecology and Obsterics, Shengjing Hospital and First Clinical Hospital of China Medical University, from Jul 2005 to Dec 2007.They had been diagnosised correctly by routine pathological examination .None of the patients had received radiotherapy, chemotherapy, hormonal therapy and other treatment before operation.2. Methods1. To inspect the protein expression of SULT1E1 by Western-Blot test, comparing the difference in different tissues.2. To inspect the expression of the mRNA of the SULT1E1 by RT-PCR, comparing the difference in different tissues.3. Statistical AnalysisSPSS 13.0 software was employed to analyze all data. Staistical evaluation is performed using One Way ANOVA and Independent-Samples Test, using SNK on pairwise compairing. P≤0.05 is considered as statistical significance. P≤0.01 means the difference marked.Results1.The content of SULT1E1 protein expression in normal endometrial proliferation,secretory period and shrink endometrial tissue was significantly (P <0.01). The protein content was significantly between secretory and proliferation phase (P <0.01). The difference was considered as statistical significance between secretory and shrink endometrial (P <0.05), but the proliferation and shrink endometrial in no difference (P> 0.05)2.The content of SULT1E1 protein expression in normal endometrium was the highest, and decreased in atypical hyperplasia and endometrial adenocarcinoma tissues. The difference among the three groups was very marked(P<0.01). The difference was marked between atypical hyperplasia or endometrial adenocarcinoma and normal endometrium.3.The content of SULT1E1 mRNA expression in normal endometrial proliferation,secretory period and shrink endometrial tissue was significantly (P <0.01). The protein content was considered as statistical significance between secretory and proliferation phase or shrink endometrial (P<0.05), but the proliferation and shrink endometrial in no difference (P> 0.05).4.The content of SULT1E1 mRNA expression in normal endometrium was the highest, and decreased in atypical hyperplasia and endometrial adenocarcinoma tissues. The difference among the three groups was very marked (P<0.01). The difference was marked between atypical hyperplasia or endometrial adenocarcinoma and normal endometrium.5.In endometrial adenocarcinoma tissues with different clinical stage, pathological, muscular invasion and so on, the expression level of the mRNA and protein of SULT1E1 were of no difference(P>0.05).6.In endometrial adenocarcinoma, SULT1E1 mRNA and protein in the ER-positive was significantly higher than the ER-negative with significant differences (P<0.01).Conclusions1.The content of SULT1E1mRNA and protein at different times normal endometrium, secretory period was the highest, which indicated progesterone may be induce SULT1E1 expression.2.There were expression of SULT1E1 mRNA and protein in the normal endometrium, atypical hyperplasia endometrial and endometrial adenocarcinma tissues. The expression of SULT1E1 mRNA and protein in endometrial adenocarcinaoma and atypical endometrial was lower significantly than that in normal endometrium, which indicated SULT1E1 may be related with the tumorigenesis of endometrial adenocarcinoma.3.In endometrial adenocarcinoma tissues with different clinical stage, pathological grade and muscular invasion, the expression level of the mRNA and protein of SULT1E1 were of no difference, however pathological grade responded the essence biological characteristic of tumor, which indicated SULT1E1 wasn't index forendometrial adenocarcinma.4. In endometrial adenocarcinoma tissues, SULT1E1mRNA in the organization of ER-positive was significantly higher than the ER-negative, prompted SULT1E1 physiological significance was the regulation of estrogen on the role of ER-positive.