Studies On The Mechanisms Of Compatibility Safflower Seed Oil To Enhance The Resistance Capacity Of Brain Cells And Mitochondria Against Free Oxygen Radical Mediated Cell Damage

Objective:â‘ To prepare of compatibility safflower seed oil composed ofα-linolenic acid and linoleic acid in different proportion;â‘¡Establishment of a brain cell(PC12 cell line) damage model(apoptosis) induced by H₂O₂ stimulation,and studies on the mechanisms of compatibility safflower seed oil to enhance the resistance capacity of brain cells against free oxygen radical mediated cell damage.â‘¢To study the capacity of compatibility safflower seed oil against lipid overoxidation in a mitichondrian damage system induced by H₂O₂/Fe²⁺.Methods:â‘ To prepare compatibility safflower seed oil,and determine theα-linolenic acid and linoleic acid contents by GC method(gas phase chromatography);â‘¡To establish a free oxygen damage(apoptosis) model of PC12 brain cells,and to analyze the drug treatment of compatibility safflower seed oil in different dosage by methods of MTT,fluorescence imaging and flow cytometry to evaluate the relationship between cell activity and the dose-effectâ‘¢To prepare mitochondria from rat brain by modified methods by Clark and establish an oxidative damage system with H₂O₂/Fe²⁺, and determine brownish lipid,membrane fluidity and ATPase activity before and after treatment of compatibility safflower seed oil.Results:â‘ The contents ofα-linolenic acid and linoleic acid were about 62.49% and 22.91%,respectively,to meet the requirements of compatibility safflower seed oil;â‘¡The lowest dosage to establish the H₂O₂-induced cell damage was 200μM;the lowest dosage of compatibility safflower seed oil to make a significant resistance of PC12 cells against free oxygen mediated damage was 0.3mg/ml.â‘¢The ability of rat mitochondria to inhibit lipid overoxidation was significantly enhanced when the dosage of compatibility safflower seed oil kept at 0.3-0.5mg/ml.Conclusions:â‘ The method to prepare compatibility safflower seed oil proved to be reproducible and feasible;â‘¡H₂O₂-induced cell damage model could be applied for the drug treatment with compatibility safflower seed oil,and was reproducible and stable.â‘¢The ability of compatibility safflower seed oil to resist free oxygen radical mediated cell damage maybe achieved by indirectly enhancing self-defense capacity of brain cells, instead of the direct contact between the drug and the free oxygen radicals. Compatibility safflower seed oil may work through the sub-cellular compartment-mitochondria by enhancing the anti-oxidation capacity of the cell.