Primary Investigaion Of The Relationship Between Glucocorticoid Induced Leucine Zipper (GILZ) And Inflammatory Reaction

OBJECTIVE: Primary investigation about the mechanism of the glucocorticoid induced leucine zipper(GILZ)in inflammatory reaction.METHODS: Human monocyte cell line (THP-1) was divided into two groups and cultured in nonserum medium, in one group treated with dexamethasone. Twelve hours later total RNA and total protein were abstracted in both two groups. The mRNA encoding for expression of GILZ was detected by the methods of the RT–PCR. Protein expression of NF- B and AP-1 were surveyed by Western blotting. Peripheral blood of 10 trauma patients were collected and the leukocyte were isolated. The leukocyte were divided into two groups and cultured in nonserum medium, in one group treated with dexamethasone. Twelve hours later total RNA and total protein were abstracted in both two groups. The mRNA encoding for expression of GILZ was detected by the methods of the RT–PCR. Protein expression of NF- B and AP-1 were surveyed by Western blotting.RESULTS: Stimulated by dexamethasone, the expression of GILZ mRNA was increased in the stimulation group, this result both in THP-1 cells and the leukocyte of trauma patients(P<0.01). Whereas, protein expressions of NF- B and AP-1 were decreased in the stimulation group(P<0.01).CONCLUSIONS: The expression of GILZ gene was up-regulated by glucocorticoids. Overexpression of GILZ inhibited NF- B and AP-1 activity, it suggested that GILZ possess anti-inflammatory function.