| Objective: To investigate the effects of sodium arsenic(NaAsO2) on human lymphocyte cells activities,anti-oxidation system and RNA of CAT. Methods: Lymphocyte were incubated with NaAsO2 in different does for 24h,48h,72h.MTT reduction assay was used to observed cells activities.Glutathion(GSH), Superoxid(SOD),Malondialdehyde (MDA) were measured and analyzed by enzyme linked immuno- absorbent assay. Catalase(CAT) was measured by ultraviolet spectrometry. RT-PCR was used to detect the RNA expression of CAT. Results:1. Compared with the control group, when the cells were in 2,5μmol/L NaAsO2 groups, the activity of lymphocytes increased, and in the does of higher than 10μmol/L groups,the activity of lymphocyte decreased;2. When the cells were in 2～10μmol/L NaAsO2 groups, the level of GSH and the activity of SOD and CAT increased significantly.While the level of MDA decreased significantly. Moreover,in high does groups(15～60μmol/L) , the results were oppositive,and showed a interaction of time and does;3. GSH has positively correlation to SOD(r=0.977 , P < 0.01)and negatively correlation to MDA(r=-0.956 , P < 0.01), meanwhile,MDA has negatively correlation to SOD(r=-0.924,P<0.01);4. CAT has positively correlation to GSH(r=0.784,P =0.000) and SOD(r=0.765 , P=0.000).CAT has negatively correlation to MDA(r=-0.814,P=0.000).5. If the cells were treated with 2~10μmol/L sodium arsenite,RNA of CAT were up regulated,and if the cells were treated with 15~60μmol/L sodium arsenite,RNA of CAT were down regulated and and in a doesge dependent fashion. Conclusions: This study suggests that the lymphocyte activities can be inhibited and the level of mRNA of CAT was decreased by NaAsO2 in the does higher than 15μmol/L.Arsenic can increase the level of aiti-oxidation action by decreasing the levles of anti-oxidation matarial and the activities of antioxidase. Catalase may influence the risk of arsenism. Catalase can be considered as endemic arsenic susceptibility markers. |
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