The Study Of The Regulating Lipid Effects Of Compound Curcuma

1 ObjectiveThe Compound Curcuma microcapsules is by the Curcumin which and the pipeline two kind of monomer ingredients which withdraws from the traditional Chinese medicine Curcuma rhizome withdraws from the long pepper cob according to the certain proportion blend composition traditional Chinese medicine compound prescription slow release preparation. Had the research to indicate that the Curcumin and the pipeline have the regulating lipid effects; But, the Curcumin itself exists some questions, for example in water solubility low, when the small intestine absorbs has the biotransformation, the stability badly and so on, causes its oral administration blood medicine density to be low, the biological exploitability is small, was big with the dose, has limited the clinical promoted use, in view of these questions, in the Curcumin and the liver, the intestines the glycuronic acid union inhibitor - - pipeline came in handy,suppresses in the intestines the Curcumin glucosone acidification function; And uses the microencapsulation technology, increases its stability, the promotion absorption, enhances the biological exploitability. This article through is true to the different kind examines animal's experiment, the observation compound prescription turmeric yellow to the animal blood fats level influence, and preliminary discussion compound prescription turmeric yellow accent blood fats function mechanism.2 Methods2.1 Takes 84 NIH mice, is half and half female and male, tests under the environment the convention raises 3 days later, stochastically divides into 7 groups, namely blank control group, model group, Fenofibrate group (Fenofibrate), Chinese hawthorn group (Chinese hawthorn essence), compound compound Curcuma microcapsules high monitoring team, compound Curcuma microcapsules monitoring team, compound Curcuma microcapsules low monitoring team, each group of 12 ones. Besides the blank control group and the model group, others give the medicine separately, for the dose is 20ml/kg, daily one time, continuously 7 days; After before end time for medicine, 16h, besides the control group, other each group of tail passages through which vital energy circulates injects Triton WR-1339 400mg/kg, fasts to be unable to restrain the water. After end time for medicine 1h, various mice pick the eyeball to draw blood, 1000rpm,10min, separation blood serum, determination blood serum cholesterol (TC), triglyceride (TG) content. Compared with each group of differences.2.2 Takes SD big mouse 70, is half and half female and male, in tests under the environment to give the normal foundation feed convention to raise for 1 week, then stochastically divides into 7 groups stochastically: The blank control group (for ordinary feed), the model group (for high fat feed), the Fenofibrate control group, the Chinese hawthorn control group, the Compound Curcuma microcapsules high monitoring team, the Compound Curcuma microcapsules monitoring team, the Compound Curcuma microcapsules low monitoring team, each group of 10 ones, is half and half female and male. Free potable water; Besides gives the high fat feed, every night supplements the normal foundation feed. At the same time, fills the stomach respectively for the medicine group according to the 10ml/kg dosage to the medicine, the blank group and the model group gives the same isometric the distilled water, daily 1 time, continuously 8 weeks. Before end the time the animal fasts 12h for the medicine, after end time for medicine 1h, the processing animal carries on the following determination:(1) The blood serum lipid determination, the big mouse eye socket draws blood, separation blood serum, determination blood serum TC, TG, high density lipoprotein -cholesterol (HDL-C), low density lipoprotein - cholesterol (LDL-c), third dial (MDA), Guguangantai peroxidase (GSP-PX), hyperoxide mutase (SOD), lipoprotein fat enzyme (LPL), liver fat enzyme (HL), total fat enzyme.(2) The liver pathological change inspects: The execution animal, opens the abdominal cavity, observes the liver luster, takes out the liver, weighing and calculates the liver coefficient; The partial liver organization uses in the pathology slice inspection from liver left Ye Jianqu. liver coefficient = liver weight/big mouse body weight.(3) Liver organization lipin determination: Takes the liver left leaf partial liver organization weighing, the cuttings, grinds the refining, adds the 9ml chloroform -methyl alcohol intermixture according to each gram sample (1:1), shakes fully in the test tube, the extraction lipin time is 24h, the centrifugal supernate measures TG, TC and so on.2.3 Takes New Zealand rabbit 42, in tests under the environment, gives the normal foundation feed convention to raise for 1 week, then draw an item at random 6, give the normal foundation feed, other 36 give high-grade feed 100g in the morning every day, every night supplements the normal foundation feed again, continuously 4 weeks. 4th week-long last day ago all domestic rabbits fast 12h, the ear reason vein draw blood, determination blood serum cholesterol content. Then carries on the grouping by the blood serum cholesterol's level (blank group exception), respectively is the model group, Fenofibrate group, Chinese hawthorn fine group, compound prescription turmeric yellow high monitoring team), monitoring team, low monitoring team..After the grouping, the blank group and the model group fill the stomach to give the same dosage the distilled water, other give according to the dosage fill the stomach, daily 1 time, continuously 8 weeks. Before end the time fasts 12h for the medicine, after end time for medicine 1h, the processing animal carries on the following determination:(1) The blood serum lipin determination, before the ear median artery draws blood (draws blood, fasts 12h) to separate the blood serum, determination blood serum TG, TC, HDL-c, LDL-c.(2) The aorta pathological change's pathology examination, takes out the heart to qia the artery branch place aorta, splits only the external coat periplast and periphery the fat, by the abdomen linea mediana longitudinal incision, is fixed with 10% formalin solution, the Sudanese III dyeing, after the arch of the aorta decides as the selection, the paraffin section, HE dyes, the observation organization changes.(3) The liver pathological change inspects, opens the abdominal cavity, observes the liver luster, takes out the liver, weighing and calculates the liver index; The partial liver organization uses in the lipid determination and the pathology slice inspection from liver left part.3 Results3.1 To mouse's function, (blank) the group compares with the comparison, model group blood serum TC, TG obviously elevate(P<0.01); After giving the medicine treatment, with the model group compares, each treatment group animal's TC, TG the level obviously reduces(P<0.01); Compares with the model group, the compound prescription turmeric yellow high dose, the dosage and the low dose can reduce hyperlipemia mouse blood serum TC, TG obviously(P<0.01).3.2 To the big mouse's function, demonstrated the compound prescription turmeric yellow can reduce blood serum TC(p<0.01), TG(p<0.01),LDL-C(p<0.01),MDA(p<0.01) and the liver index(p<0.01), and present certain dosage relations; Can reduce liver TC, the TG; Can elevate HDL-C(p<0.05),SOD(p<0.01),GSH-PX(p<0.01),LPL(p<0.01),HL(p<0.05) and total fat enzyme (p<0.01);3.3 To domestic rabbit's experiment, demonstrated the compound prescription turmeric yellow can reduce blood serum TC(p<0.05),TG(p<0.01),LDL-C(p<0.01) and the liver index(p<0.01) ,and can elevate HDL-C(p<0.05).4 Conclusion4.1. The Compound Curcuma microcapsules to the hyperlipemia mouse, the big mouse and the domestic rabbit has the obvious adjustment lipin metabolism function, can reduce blood serum TC, TG, LDL-c and liver TC, the TG content; Elevates HDL-c the content; Elevates blood serum HL, LPL, the total fat enzyme content, enhances its active function.4.2. The Compound Curcuma microcapsules can elevate hyperlipemia big mouse blood serum SOD, the GSP-Px content, enhances its active function, reduces MDA.4.3. The Compound Curcuma microcapsules on rat liver fat are invasion inhibition. Three-dose group liver tissue than fat drops to reduce the model group, are scattered in the red blood cells, liver cells but no structural damage, no inflammatory infiltration.4.4. The Compound Curcuma microcapsules on rabbit aortic atherosclerosis inhibited. Three-dose group arterial pipe wall than the fat drops to reduce the model group.