| Objective:To study the effect and action of Art on tumor in vitro and explore its possible molecular mechanism,and also provide experimental evidence andthe oretical basis in order to clinical application.Methods:①Cell culture and MTT were performed to observe the effect and action of Art on tumor.②Hoechst,Flow cytometry,ELISA,DNA LADDER,IHC were performed to observe the mechanism of Art on tumor.Results:①The effect of Art was widely on tumor,which markly suppressed the proliferation of tumor cell 7901,A540,K562,A2780,HEPG-2,KBV200 and their IC50were(81.9±4.6),(78.4±5.1),(59.1±2.8),(93.7±4.3),(75.3±4.1), (113.3±7.2)μg/ml in 48h.②Art(50-200μg/ml)could inhibit HEPG-2 cells proliferation,their IC50 were(103.1+8.6),(75.3+4.1),(65.7+6.0)μg/ml in24h,48h,72h.③By adding iron,which could not enhance the effect of Art.④When Art(10μg/mL,20μg/mL)and the 5-Fu(2.5μg/mL,5μg/mL)joint use,which showed obvious synergies.⑤The results of Hoechst,Flow cytometry,DNA LADDER,which were compared with the results of control group,artesunate induced apoptosis signifficantly(p＜0.01).⑥Art groups were compared with the control group,the concentrations of caspase-3 in art group were much higher(p＜0.01),and the percentage of bcl/bax changed significantly(p＜0.01).⑦Art groups were compared with the control group compared with the control group,the distribution of cell cycle in art group changed significantly an d the cyclin G2/M changed significantly(p＜0.05).Conclusions:①Art can inhibit the proliferation of HEPG-2 significantly, When Art and the 5-Fu joint use,showing obvious synergies,possible to develop the supplementary drugs of anticancer drug;When Art and the iron joint use,showing no obvious effect.②Art can induce apoptosis,its mechanism may damage DNA,activate caspase-3,affect percentage of bcl/bax and so on.③Art can affect the distribution of cell cycle,induce G2/M phase arrest. |
PDF Full Text Request