Study On The Protective Effects Of Brain-Derived Neurotrophic Factor On Bilirubin-Injured Neurons

Objective : To observe the apoptogenic effect of bilirubin-injured neurons and investigate the protective effects of brain-derived neurotrophic factor(BDNF) against the injury. In order to supply some experimental bases for clinical application.Methods : The model of bilirubin-injury was established, cortical neurons cultured for 8 days were randomly divided into three groups: control group, bilirubin-injured group ,BDNF group. The neurons viability were detected with the method of MTT. The apoptosis of neurons was examined by fiow cytometry and phase contrast miscroscope..Results In comparison with control group , Bilirubin inhibited the cell viability and increased the apoptosis rate and death of cortical neurons, BDNF reversed upper progress, ameliorate the ratio of apoptosis (P<0.05) greatly. The protective effect of BDNF after the injury reaching a certain degree( > 24h)would significantly weaken. The protective effect of different dose BDNF were different. (P<0.05).Theprotective effect of middle dose of BDNF(50ng/ml) was very significantly.Conclusion Bilirubin damages cortical neurons and induced apoptosis .BDNF offer neuroprotective effects to bilirubin-injured cultured rat cortical neurons. But the neuroprotective effects correlate with dose of BDNF and time of action. If the major dose BDNF has deleterious effect hould be further investigated.