The Relationship Between Matrix Metalloproteinase-9 And High Sensitive C-reactive Protein With Stability Of Coronary Artery Atheromatous Plaque

Objective: To study the relationship between matrix metalloproteinase-9 and High sensitive C-reactive protein with stability of coronary artery atheromatous plaque.Methods: Sixty-seven coronary heart disease patients who were cured between October, 2007and January, 2008 in our hospital were enrolled. The mean age was (59.48±5.70)years old, 43 males and 24 females. There were 23 stable angina pectoris patients, 22 unstable angina pectoris patients and 22 acute myocardial infarction patients among them. Twenty paroxysmal supraventricular tachycardia patients who were cured during the same time in our hospital without structural heart disease(but with good heart function) were enrolled as control group, the mean age was (44.35±5.41)years old, 10 males and 10 females. To draw 2 ml vein blood on an empty stomach, put it into heparin anticoagulation tube (dissolved blood was abandoned), and take it to centrifuge for 10 minutes with the speeds 2000 circles/min in. Take 200μl supernatant into Et tube, and put it into -20℃refrigerator. To use ELISA kit to detect the matrix metalloproteinase-9 concentration of stored examples according to description. High sensitive C-reactive protein concentration was detected by the probation department.Results:1. The MMP-9 concentration(558.97±326.14) ng/mL of general CHD group is significantly more than it (156.15±64.19) ng/mL of control group(p<0.01). The MMP-9 of SA group, UA group and AMI group are (276.04±95.19) ng/ml, (513.14±136.72) ng/ml and (900.59±306.56) ng/ml, and are all more than them of control group(p<0.05, p<0.01,p<0.01). The MMP-9 of UA group is more than it of SA group(p<0.01). The MMP-9 of AMI group is more than it of SA and UA groups(p<0.01) 2. The HS-CRP concentration(5.11±2.94) mg/Lof general CHD group is significantly more than it (156.15±64.19)ng/mL of control group(1.68±0.76) mg/L(p<0.01). The HS-CRP of UA group and AMI group are (4.71±1.5)mg/L and(8.23±2.38)mg/L, and are all more than them of control group(p<0.01,p<0.01). The HS-CRP of UA group and AMI group are significantly more than it of SA group(p<0.01). The HS-CRP of AMI group is significantly more than it of UA group(p<0.01).3. The inference of MMP-9 is better than HS-CRP for stability of coronary artery atheromatous plaque(p<0.01). The result is tested by Logistic regression analysis.Conclusions:1.The MMP-9 and HS-CRP of general CHD group is significantly more than them of control group(p<0.01). The MMP-9 and HS-CRP of UA group is significantly more than them of SA group(p<0.01), and they of AMI group is significantly more than them of UA group(p<0.01). MMP-9 has more considerable relationship with stability of coronary artery atheromatous plaque than HS-CRP.2 There is positive correlation between MMP-9 and HS-CRP in the blood. The level of MMP-9 and HS-CRP reflects the inflammation degree of atheromatous plaque, and cues that anti-inflammatory drugs should be used.