The Expression And Clinical Significance Of C-erbB-2,Bcl-2,Bax And P53 In Colorectal Cancer

Objective: To analyze the expression and clinical significance of c-erbB-2,bcl-2,bax and p53 in colorectal cancer by detecting their expression in cancerous and adjacent tissues(> 5cm from the cancerous tissue).Methods: Examples of 75 cases of colorectal cancer who were treated in tumor hospital of Liaoning province from December 2007 to March 2008 ,Male 44,female 31,aged from 30 to78 years old and 54±4.6 for average. Each part respectively in cancerous and adjacent tissues (> 5cm from the cancerous tissue). The clinical stages were identified prior to operation. All patients have not received any treatment before. The expression of c-erbB-2,bcl-2, bax and p53 protein in all case were detected by immune histochemistry (S-P).The photographs of S-P were taken by microscope and the data were examined by chi-square test .Results: The positive expression of c-erbB-2 protein in this group of 75 cases of cancerous tissues was 50.7 % (38/75), with performance of yellowish brown pellet in the cell membrane.adjacent tissues was negative;The positive expression of bcl-2 protein in this group of 75 cases of cancerous tissues was 61.3% (46/ 75), Adjacent tissues was 10.7% (8/ 75) ,with performance of yellowish brown pellet in cytoplasma and cell membrane, distribution of focus or diffuse; The positive expression of bax protein in this group of 75 cases of cancerous tissues was 46.7 % (35/ 75), adjacent tissues was 81.3% (61/ 75), existing in the form of the yellowish brown pellet in the cytoplasma, distribution of focus or diffuse. The positive expression of p53 protein in this group of 75 cases of cancerous tissues was 52.0%(39/ 75), adjacent tissues was negative ,mainly located in the cell nucleus,with clear brown color of cell nucleus; The statistical analysis demonstrated the expression of c-erbB-2 had no significant differences with age,gender,and tissue type, only had significant differences with size ( P<0.05 ) ;Expression of bcl-2 had significant differences with metastasis of lymph node, Dukes' staging(P<0.05); The expression of bax and the p53 protein had no significant differences with the clinical index of colorectal cancer (P > 0. 05); the four gene expressions had a positive rate between cancerous and adjacent tissues (P<0.05).Conclusion:1, The expression of c-erbB-2 had no significant differences with age,gender,and tissue type metastasis of lymph node,Dukes' staging, only had significant differences with size;2, Expression of bcl-2 had significant differences with metastasis of lymph node , Dukes' staging;3, The expression of bax and the p53 protein had no significant differences with the clinical index of colorectal cancer;4, The four gene expression had a positive rate between cancerous and adjacent tissues.