Study On The Analysis Of Alkaloids And Nucleosides In Bulbus Fritillariae

Bulbus Fritillariae,a well-known traditional Chinese medicine (TCM).It is derived from the bulbus of various Fritillariae species.And it has been used as antitussive and expectorant drugs for centuries. Various chemical and pharmacological studies on Fritillariae have demonstrated that isosteroidal alkaloids are the main bioactive ingredients in Fritillariae.Currently,some naturally occurring non-alkaloid constituents in aqueous extracts of Fritillaria species have attracted much attention for their other various pharmacological activities such as remarkable antibacterial actions,anti-hypertension effect and platelet aggregation inhibition.This article mainly established methods for study on the alkaloids and nucleosides in the bulbus Fritillariae by high performance liquid chromatography coupled with electrospray ionization mass spectrometry or ultraviolet spectrometry.The studies in this thesis are summarized as follows:1.An HPLC-ESI/MS method was developed for analysis of alkaloids in Bulbus Fritillariae.And this method has been utilized to determine and compare of the content of verticine and verticinone in F.cirrhosa D.Don and F.thunbergii Miq.This method can be applied to quality supervise for F.cirrhosa D.Don and F.thunbergii Miq.in the market. 2.Using the LC-ESI-MS~n technology for discovering nucleosides in Bulbus Fritillariariae cirrhosae and Fritillariariae thunbergii Miq.. Because this technology can provide information structure of compounds on line and has high sensitivity.Adenine,uridine and guanosine in Fritillariae species are firstly ascertained.A LC-ESI-MS~n method for the species discrimination of Bulbus Fritillariariae cirrhosae and Fritillariariae thunbergii Miq.was reported based on the nucleosides components.3.LC/MS method has advantages of high sensitivity and short analysis time,etc.So it can be utilized to identify and analyze active components in TCM.A rapid,high sensitivity and reproducible HPLC-UV-ESI/MS technique was developed for the quantitative analysis of nucleosides components in Bulbus Fritillariariae cirrhosae and Fritillariariae thunbergii Miq..It is also suitable for the detection and analysis of nucleosides components in other plants.4.Purification and separation of uridine,guanosine,adenosine and thymidine in F.thunbergii Miq.by preparative HPLC were discussed. The method of preparation is simple,feasible and effectively economizes organic solvent.