The Correlation Between Ezrin Protein,CD44-V6 And The Invasion,Metastasis In Laryngeal Squamous Cell Carcinomas

Objective:To investigate the localization of Ezrin protein and the expressions of Ezrin protein and CD44-V6 levels in laryngeal squamous cell carcinoma(LSCC),to explore the role and relevance of Ezrin protein and CD44-V6 in invasion and metastasis of LSCC ,and then search for tumor markers which would be a new target to evaluate the metastasis and prognosis of LSCC.Methods:1. Collect 81 cases of LSCC paraffin samples(LSCC group)and the corresponding margin laryngeal mucosa paraffin samples(control group),and investigate the localization of Ezrin protein and the expression of Ezrin protein levels by immunohistochemistry(S-P).2. Detect the expressions of Ezrin protein and CD44-V6 in tumor core region(TC),tumor transition region(TR)and tumor free region(TF)of 36 cases of fresh LSCC by Western-blot.Results:1. Ezrin protein immunoreactivity was apparent in the cell membrane of most adjacent normal laryngeal mucosa cases, while it was found to be mainly diffused in the cytoplasm in most poorly differentiated while it was preferentially localized both in the membranous region and the cytoplasm in most moderately differentiated and well differentiated LSCC. The positive rates of Ezrin expression were 91.36%(74/81)and 46.91%(38/81)in LSCC group and control group respectively. The expression of Ezrin was closly related to lymph node metastasis,tumor grading and tumor differentiation.2. Western-blot results showed that the expressions of Ezrin protein and CD44-V6 in TF,TR and TC of 36 cases matching samples were increased progressively;The expressions of the both were concerned with the Tumor stage, Node stage and Pathology grade of LSCC; There were closely links between Ezrin protein and CD44-V6(r =0.633,P<0.01).Conclusion:1.Ezrin protein had three expression patterns in laryngeal tissues: membrane distribution, cytoplasm distribution and mixed distribution. Ezrin protein had a tendency to translocate from the membrane to the cytoplasm in the progression from normal epithelium to invasive carcinoma of larynx. The result suggested that Ezrin protein might have a key role in the invasion and metastasis of LSCC.2.Ezrin protein and CD44-V6 were abnormally highly expressed in LSCC, and there was great relevance in invasion and metastasis of LSCC.3.The combined detection of Ezrin and CD44-V6 may have significant prognostic value for assessing the degree of malignancy and potential lymph node metastasis of LSCC, to provide theory basis for the gene target therapy.