| Objective To study the biological characters of chondrocytes cultured in vitro on typeâ… collagen compound polylactic acid scaffolds such as the tendency of growth and stability of phenotype. Method We had constructed tow groups of three diamensions cultured systems: polylactic acid scaffolds and typeâ… collagen compound polylactic acid scaffolds in the culture process, observe each group's cytobiologic transformation of chondrocyte .Result The chondrocytes cultured in polylactic acid scaffolds,the 5th generation partly showed negative immunohistochemistry expression of typeâ…¡collagen, the 8th generation total showed negative immunohistochemistry expression of typeâ…¡collagen. The chondrocytes cultured in the typeâ… collagen compound polylactic acid scaffolds, the 8th generation still showed positive immunohistochemistry expression of typeâ…¡collagen, H-E stain showed that the form of the chondrocyte was round and maintained the excellent differentiated phenotype. Conclusion The typeâ… collagen compound polylactic acid scaffolds can maintain the normal growth of chondrocytes by improving phenotype stability and increase the matrix metabolism of chondrocytes. It suggested that the typeâ… collagen compound polylactic acid scaffolds 3D culture in vitro systems might be an effective way to researchs of articular cartilage tissue engineering.
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