A Study Of The Relationship Of Polymorphism Of Peroxisome Proliferator Activated Receptor Gamma Gene(PPARγ) And Osteoporosis In Aged Male

Objective To study the relationship of the single nucleotide polymorphisms(SNP)of exon6 C161→T of peroxisome proliferators activated receptorγ(PPARγ)gene and osteoporosis in aged male.Methods Genomes DNA were extracted from peripheral white blood cells and PCR-RFLP was used to analyze the gene frequency distribution in non osteoporosis(NOP)group and osteoporosis(OP)group in old male.Bone mineral density of lumbar and the upper thighbone(triangle of big rotor, neck of thighbone and Ward's)were measured by dual energy X-ray absorptiometry.Serum osteocalcin were measured by ELISA.Results The exon6 of PPARγhave three genotype(CC,CT and TT).The distribution frequency of genotype accord with Hardy-Weinberg law.The gene frequency of T allete in osteoporosis were higher than that in non osteoporosis.Compare with the control group,OP have a lower level of serum bone glaprotein and bone mineral density.The bone mineral density in the genotype of CT and TT were lower than that in the genotype of CC.Conclusion Our study shows the SNP of 6th exons of PPARγmay relate to osteoporosis in old males.T allele of PPARγmay be a impressionable factor of osteoporosis in old males.PPARγmay be a candidate gene of osteoporosis in old males.