Effects Of TENS On Urinary Blaader Voiding Dysfunction In Diabetic Cystopathy Rats

Objective To investigate the effects of Transcutaneous Electrical Nerve Stimulation(TENS)on the urinary blaader voiding dysfunction in diabetic cystopathy rats.Methods SD rat model of diabetic cystopathy rat was established by the injection of STZ in abdominal cavity.The rats were randomized into the DM ES(DM with electrical stimulation,n=18)group,DM(diabetes mellitus,n=18) group and normal control group(n=14).TENS was performed on rats in the DM ES group in the 10th week after the establishment of diabetic rats model (stimulation parameter:intensity--31V,density--31Hz;two pairs of electrode was place on the projective area on the abdomen of the bladder respectively), but those in the control group underwent shamoperation.After the stimulation was continuing for 3 weeks,The bladder wet weight,volume threshold for micturition,post-voided residual volume,bladder residual volume/bladder capacity(R%),the max micturition pressure,the changes of the detrusor strip contractility and bladder muscle ultrastructure were detected.The expression of CGRP mRNA in each rats' DRG was measured by RT-PCR,The expression levels of CGRP of Bladder and DRG were detected by Western-blot.Results Compared with DM group,the R%(DM group:42.83±8.71;DM ES group:32.82±6.72)and volume threshold for micturation(DM group:1.40±0.40ml;DM ES group:0.93±0.26ml)were significantly reduced while the max micturation pressure of the bladder(DM group:19.49±6.51 mmHg; DM ES group:30.0±3.84mmHg)were significantly increased in DM ES group after electrical stimulation.Histopathological studies showed that the detrusor cells expressed compensatively hypertrophy,intracellular collagen fibers increased obviously and mitochondra swelled in the bladder muscle unltrastructure of diabetic rats.Electrical stimulation could reduced markedly the compensated hypertrophic cells,intercellular collagen gibers and protect mitochondria in the bladder muscle ultrastructure of DM ES group,The content of the mRNA of CGRP of DRG in DM ES group is enhanced than that in DM group.The expression levels of CGRP of Bladder and DRG in DM ES group is increased than that in DM group.Conclusion These experiment result demonstrate that the TENS can promote the voiding in the DCP(diabetic cystopathy)rats,TENS may enhance the expression levels of CGRP of DRG and bladder in DCP,Which react in the bladder through the effective conduction,improve the bladder fullness,restore the initiate factor of the micturition.Otherwise TENS also ameliorate the configuration and function of ultrastructure,regulate the intracellular Ca²⁺ concentration to facilitate bladder contractility.TENS maybe accelerate the restoration of the nerver through enhace The expression levels of NGF or the injury/regeneration related gene.