Objective To study the effect of ultraviolet(UV) radiation on the mRNA expressions of ubiquitin and ubiquitin-activating enzyme in rat lens in vitro.Methods The mRNA expression levels of ubiquitin and ubiquitin-activating enzyme in rat lens after UV-radiation were determined by the real-time quantitative RT-PCR (SYBR Green) method.Results The results showed that after rat lenses were exposed to UV-irradiation, the mRNA expression levels of ubiquitin and ubiquitin-activating enzyme began to decrease and reached the lowest levels after 1 hour .The recovery process showed after 48 hours of recovery, their mRNA expression levels reached nearly the normal level, compared with the control group there has been no noticeable difference. However, the mRNA expression level of GAPDH kept unchanged.Conclusions These results suggested that UV-irradiation might inhibit the activity of ubiquitin - proteasome pathway by reducing the gene expression levels of ubiquitin and ubiquitin-activating enzyme. It might affect the degradation of lens abnormal protein.
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