The Experiment Study For Influences Of Mild Hypothermia On Expression Of Thrombin Receptor-1 And Apoptosis Inducing Factor Following Intracerebral Hemorrhage

Objective To investigate the effect of mild hypothermia on the expression of thrombin receptor-1 and apoptosis inducing factor following incerebral hemorrhage in rats and clarify the mechanism of hypothermia on formation.Methods fifity four rats were randomly divided into three groups:the normal saline(NS) group,the intracerebral hemorrhage(ICH)group,in which autologous arterial blood were stereotaxically injected into right caudate nucleus,the mild hypothermia(HT)group,in which the rats were given mild hypothermia after the injection of blood.Immunostaining was used to detecting the expression of PAR-1 and AIF at 24h,72h and 7d after ICH. Meanwhile,Dry-Wet weight was used to measured brain water of the cerebral hemorrhage group and the mild hypothermia group.Resultâ‘ The expression of PAR-1 and AIF began to increase at 24h,peaked at 72h, decreased at 7d after ICH,meanwhile they were higher than the saline group;â‘¡The expression of PAR-1 and AIF in the mild hypothermia group were significantly lower than in ICH group at 24h,72h and 7d time-points;â‘¢The brain water content began to attenuate at 24h,peaked at 72h and decreased at 7d after ICH;â‘£The brain water content of mild hypothermia group at three time-points were lower than the ICH group.Conclusion The expression of PAR-1 and AIF increased after ICH,so they maybe play an important role in the formation of brain edema and the apoptosis of neuron cells,and participated in the brain injury after ICH.Mild hypothermia can alleviate brain damage after ICH by inhibiting the expression of PAR-1 and AIE...