| Objective:(1)To explore Monocyte chemoattractant protein-1 expresses in rat experimental retinal detachment at different times;the relationship between MCP-1 and photoreceptor apoptosis in retinal detachment.(2)To explore blocking the expression of MCP-1 in retinal detachment can diminish photoreceptor apoptosis.Methods:46 Brown Norway rats were involved in this study.(1)37 rats were builded the retinal detachment model in the right eye,and the left eye as control.Except 7 rats which were not fit to the standard model(include death, endophthalmitis and retinal hemorrhage).The left 30 rats devided into 5 groups according force-managed times(1h,6h,12h,24h,72h).Then rats were killed and the eyes were enclutered.The expression of MCP-1 in retinal detachment eyes were assessed by immunohistochemical method and cell death were quantified by TUNEL assay.Analysis the relatinship between MCP-1 and TUNEL in detached retinal by statistical software.(2)9 rats were builded the retinal detachment model both of the eyes.We injected rat anti-MCP-1 IgG subretinally during RD induction in the right eyes and PBS in the left eyes. Except 3 rats which were not fit to the standard model(include death,endophthalmitis and retinal hemorrhage).Cell death were quantified by TUNEL assay 72h after RD,since at that time the number of TUNEL-positive cells in the ONL is at the peak.Resuits(1)Immunohistochemical analysis:In normal retinal tissue,the number of MCP-1 positive cells was 3.64±1.02 under microscopy visual field.MCP-1 positive cells was 10.50±2.82,11.13±3.31,25.23±5.56,37.23±7.15,205.83±15.23 in detached retinal at 1h,6h,12h,24h,72h under microscopy visual field.Compare the expression of MCP-1 at different times of RD,every two groups had statistical variation,except the 1h and 6h group.(2)TUNEL assey:In normal retinal tissue,the number of TUNEL positive cells in ONL was 2.16±1.33/mm~2.TUNEL positive cells in ONL was 15.30±3.21/mm~2;25.11±5.31/mm~2;41.32±7.56/mm~2; 80.23±10.15/mm~2;425.83±25.23/mm~2 in detached retinal at 1h,6h,12h,24h,72h.(3)MCP-1 and TUNEL positive cell began to express remarkably after 1h of retinal detachment,then increased follow time,got the peak at 72h.The relationship between MCP-1 and TUNEL positive cell is positive correlation(r=0.905).(4)TUNEL positive cells in MCP-1 blocking group and PBS group after 72h of retinal detachment were 160.24±10.56/mm~2 and 430.15±30.42/mm~2 in ONL.There was statistical variation between MCP-1 blocking group and RD group(p＜0.05),no statistical variance between RD group and PBS group(p＞0.05).Conclution:(1)MCP-1 began to express after 1h of rat retinal detachment,then increased follow time,got the peak at 72h.(2)There is positive correlation between MCP-1 and TUNEL positive cell.(3)Blocking the expression of MCP-1 in detached retinal can diminish photoreceptor apoptosis. |
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