| Objective:To investigate changes and probable roles of Duffy antigen/receptor for chemokine(DARC)and interluekin-8(IL-8)in the development of hypertension.Methods:Study in vivo:30 patients with essential hypertension (were divided into two group for blood pressure grade:grade 1 and grade 2),and 30 normal controls enrolled in the present study.The levels of plasma IL-8 were measured by enzyme linked immunosorbent assay (ELISA)and the expression of DARC mRNA in erythrocytes was measured by realtime-polymerase chain reaction(realtime-PCR).Study in vitro:Human umbilical vein endothelial cells(HUVEC)were cultured and divided into three group:Control,AngⅡ(10-7M)and AngⅡ(10-6 M)which were treated respectively with phosphate buffered solution (PBS),10-7M and 10-6M AngⅡfor 0～24 hours.The AngⅡ-stimulated IL-8 release in the medium and IL-8 level in cell disruption fluid were measured by ELISA,and the DARC mRNA expression in endothelial cells was measured by realtime-PCR at 0,6,12 and 24h.Soft system of SPSS 13.0 was used for statistics.Results:Research in vivo:Plasma IL-8 levels were(102.25±21.01) pg/ml in EH(grade 1),and(105.93±18.93)pg/ml in EH(grade 2).Both of them were higher than control(66.70±13.37)pg/ml,which may indicated IL-8 correlates with the inflammation of hypertensin.DARC mRNA expression in erythrocytes was 2.31±0.58(relative quantitation)in EH(grade 1),and 3.13±0.84(relative quantitation)in EH(grade 2).Both of them were higher than control 1.11±0.51(relative quantitation),which may indicated DARC correlates with hypertensin.Research in vitro:AngⅡ(10-7M,10-6M)could stimulate IL-8 production and release leading to the increase of IL-8 levels in medium in time-dependent manner.IL-8 could be detected at 6h,the maximum was 12h,and decreased at 24h;Cell disruption fluid reflected the IL-8 levels in cells.They were in a time-dependent manner but not parallel with the cell culture fluid,which increased gradually during 6～24h.This may correlate with the effect of DARC for binding IL-8.AngⅡcould time-dependently stimulate the expression of DARC mRNA,which increased gradually during 6～24h.This supported the effect of DARC for binding IL-8.Conclusion:1.Plasma IL-8 levels and DARC mRNA expression in erythrocytes increased in patients with essential hypertension.2.AngⅡcan stimulate prodution and release of IL-8 and expression and DARC in endothelial cells.It indicates IL-8 and DARC may participate in and promote the inflammation of hypertension. |
PDF Full Text Request