The Study Of Immunological Escaping Mechanism Of B7-H3 And IFN-γ In Gliomas

Objective Through exploring the expressions of costimulatory molecules B7-H3 and the serum levels of IFN-γwith gliomas,To investigate the relationship between B7-H3,IFN-γand the immunological escaping mechanism of gliomas,and offer theoretical and experimental evidence for studying gliomas.Methods The protein expression of B7-H3 was examined by immunohistochemical method in 58 gliomas and 9 normal brains.The relationship between the expression levels of B7-H3 and the glioma biological behaviors was analyzed.ELISA was used to detect the level of IFN -γin the serum of the 58 patients with glioma and 20 normal people.Results Expression of B7-H3 were found in 58 gliomas,very little in 9 normal brains.The expression level of BT-H3 reduced obviously accompany with the incrasing of the grade of gliomas(P＜0.05). Each group was significantly different(P＜0.05).B7-H3 expression was not related to age and sex,There was a close correlation between B7 - H3 expression and several factors,such as histologic types and prognosis(P＜0.05).The positive rate of B7 - H3 expression in patients more than two years survival were significantly higher than that in patients less than two years survival(P＜0.05).Serum levels of IFN-γwere significantly lower in patients than in healthy control(P＜0.01),Each group was significantly different(P＜0.01).The expression of B7-H3 was significantly positive correlated with IFN-γ.(r=0.630,P＜0.01)Conclusions1.There was a close correlation between B7-H3 and glioma,the expression of B7-H3 reduced with the increase of grades of glioma, Detection of B7-H3 expression in gliomas will be beneficial to the judgment the biological behaviors and prognosis of gliomas.2.There was a close correlation between IFN-γand gliomas,the serum levels of IFN-γinduced with the increase of grades of gliomas.3.There is significant positive correlation between the expression of B7-H3 and the serum levels of IFN -γin glioma,they have significance to evaluate the biological behavior of glioma,and can provide evidence for instituting reasonable therapy scheme,judgging prognosis and assessing curative effect in clinic.