The Study On The Expression Of ERCC1,HMGB1,Bcl-2/Bax,Caspase-3 And The Correlation With Platinum-based Chemotherapy Sensitivity And Prognosis In â…¢a Stage Non-small Cell Lung Carcinoma

Objective:To research the expression of ERCC1,HNGB1,Bcl-2/Bax, Caspase-3 in patients withⅢa stage non-small cell lung carcinoma(NSCLC)as well as their correlation with platinum-based chemotherapy sensitivity and clinical prognostic significance.Method:Clinic data of 85 inpatient cases in The Cancer Hospital of Tianjin Medical University,whose final diagnosis were conformed by Mediastinoscopy,from 2004-2008,were retrospectively reviewed.Expression of ERCC1,HMGB1,Bcl-2/Bax,caspase-3 of 80 cases that were surgically resected by Mediastinoscopy NSCLC specimens(including 38 Squamous carcinomas and 42 Adenocarcinomas) were studied by immunohistochemistry.The value of the five factors' statistics analysis of the platinum-based chemotherapy sensitivity and clinical prognosis ofⅢa stage NSCLC were studied.Result:1.Results of clinical datasThe overall 1,2 and 3-year survival rates were 74.10%,62.20%and 45.50% respectively.Univariate analysis showed the main prognostic factors were primary tumor diameter,bronchial stump,post-surgery N stage,N patho-relieve,surgery and imaging category(P＜0.05).Cox regression analysis suggested that surgery,N patho-relieve and bronchial stump were the most important significant independent prognostic factor.2.The expression of ERCC1,HMGB1,Bcl-2/Bax and Caspase-3 and the correlation with platinum-based chemotherapy sensitivity inⅢa stage NSCLC.The positive rate of ERCC1,HMGB1,Bcl-2/Bax and Caspase-3 were 52.50%, 60.00%,22.50%,55.00%and 43.75%respectively in the 80 cases.The totol patho-relieve (RR)was 41.25%.The study on the expression of ERCC1,HNGB1, Bcl-2/Bax,Caspase-3 and chemotherapeutic response showed:the negative expression of ERCC1 and Bcl-2 have a significantly better patho-relieve than positive expression and the positive expression of HMGB1,Caspase-3 have a significantly better patho-relieve than negative expression.However,between the expression of Bax and patho-relieve have no significantly different.The study on the relationship between combined detection of every two proteins and chemotherapeutic response showed:the cases of ERCC1(-)/Bcl-2(-)have a significantly better patho-relieve than ERCC1(+)/Bcl-2(+),the cases of HMGB1(+)/Caspase-3(+)have a significantly better patho-relieve than the cases of HMGB1(-)/Caspase-3(-)and the cases HMGB1(+)/ERCC1(-)have a significantly better patho-relieve than the cases of HMGB1(-)/ERCC1(+).3.The expression of ERCC1,HMGB1,Bcl-2/Bax and Caspase-3 and the correlation with the prognosis inⅢa stage NSCLC.Log-rank mon-factor analysis showed that the expression of ERCC1,HMGB1, Bcl-2 and Caspase-3 have significantly correlation with the prognosis inⅢa stage NSCLC.COX multivariate regression analysis showed that the expression of ERCC1, HMGB1 and Caspase-3 were independent prognostic factors forⅢa stage NSCLC.Conclusion:1.surgery,N patho-relieve and bronchial stump were the most important significantly independent clinical prognostic factor inⅢa stage NSCLC.2. The expression of ERCC1,HNGB1,Bcl-2 and Caspase-3 were significantly independent factors with platinum-based chemotherapy sensitivity inⅢa stage NSCLC.The expression of ERCC1,HNGB1 and Caspase-3 were the most important significantly independent prognostic factors inⅢa stage NSCLC...