| Objective To investigate the correlation of protenin expression level ofβ-catenin,Axin and MMP-14 with infiltration and metastasis in esophageal squamous cell carcinoma(ESCC).Methods1 The protein expression level ofβ-catenin,Axin and MMP-14 was detected by immunohistochemistry (IHC) with Streptavidin-Peroxidase (SP) in 72 cases of ESCC, 40 cases of atypical hyperplasia tissues and 40 cases of normal mucosa.2 The protein expression level ofβ-catenin, Axin and MMP-14 in 40 cases of ESCC, 30 cases of atypical hyperplasia tissues and 20 cases of normal mucosa was quantitatively examined by flow cytometry(FCM).Results1 The expression ofβ-catenin in different lesions of esophageal tissues(IHC and FCM).The expression ofβ-catenin in 72 cases of ESCC , 40 cases of atypical hyperplasia tissues and 40 cases of normal mucosa was detected by IHC, the result showed that the aberrant expression rate ofβ-catenin protein (61.1%) in carcinoma was higher than that in atypical hyperplasia (27.5%) and normal mucosa (7.5%) (P<0.01), but with no significant difference between carcinoma and adjacent mucosa (P>0.05). The expression ofβ-catenin was not correlated with age, sex and fibromembranous invasion(P>0.05), but with cell differential degree (P<0.05), lymph node metastasis (P<0.01).The expression ofβ-catenin in 40 cases of ESCC , 30 cases of atypical hyperplasia tissues and 20 cases of normal mucosa was detected by FCM, the result showed that theβ-catenin protein content in esophageal carcinoma (558.14±57.60) was lower than that in atypical hyperplasia (665.61±73.38) and normal mucosa (732.64±65.60) (P<0.01); and theβ-catenin protein content correlated with lymph node metastasis (P<0.05) and cell differential degree(P<0.05); but not with age, sex and fibromembranous infiltration (P>0.05), which was consistent with those of IHC approximately.2 The expression of Axin in different lesions of esophageal tissues(IHC).The expression of Axin in 72 cases of ESCC , 40 cases of atypical hyperplasia tissues and 40 cases of normal mucosa was detected by IHC, the result showed that the positive expression rate of Axin protein (38.9%) in carcinoma was lower than that in atypical hyperplasia (60%) and normal mucosa (85%) (P<0.01). The expression of Axin wasn't correlated with age, sex and cell differential degree (P>0.05), but with fibromembranous infiltration (P<0.05) and lymph node metastasis (P<0.01).3 The expression of MMP-14 in different lesions of esophageal tissues( IHC and FCM). The expression of MMP-14 in 72 cases of ESCC , 40 cases of atypical hyperplasia tissues and 40 cases of normal mucosa was detected by IHC, the result showed that the positive expression rate of MMP-14 protein (62.5%) in carcinoma was higher than that in atypical hyperplasia (42.5%) and normal mucosa (2.5%)(P<0.01). The expression of MMP-14 wasn't correlated with age and sex (P>0.05) ,but with cell differential degree (P<0.05), fibromembranous invasion (P<0.05) and lymph node metastasis (P<0.01).The expressions of MMP-14 in 40 cases of ESCC , 30 cases of atypical hyperplasia tissues and 20 cases of normal mucosa was detected by FCM, the result showed that the MMP-14 protein content in esophageal carcinoma (540.44±66.80) was higher than that in atypical hyperplasia (375.33±89.92) and normal mucosa (289.68±76.85) (P<0.01); and the MMP-14 protein content correlated with lymph node metastasis (P<0.05),but not with age, sex, cell differential degree and fibromembranous infiltration (P>0.05), which was consistent with those of IHC approximately.4 There was a negative correlation between the expression ofβ-catenin and Axin protein in the esophageal carcinoma tissues (χ~2 =6.42,P<0.05)(IHC).5 There was no correlation between the expression ofβ-catenin and MMP-14 protein in the esophageal carcinoma tissues (χ~2=1.56,P>0.05)(IHC).6 There was a negative correlation between the expression of MMP-14 and Axin protein in the esophageal carcinoma tissues (χ~2=5.05,P<0.05)(IHC).7 There was no correlation between the expression ofβ-catenin and MMP-14 protein content in the esophageal carcinoma tissues (r=0.111,P>0.05)(FCM).Conclusions1 The aberrant expression ofβ-catenin in esophageal carcinoma was obviously higher than that in atypical hyperplasia and normal mucosa, which indicated thatβ-catenin could be involved in carcinogenesis. The aberrant expression ofβ-catenin was correlated with cell differential degree and lymph node metastasis, indicating thatβ-catenin might play an important role in evaluating the degree of prgession in esophageal carcinoma and might contribute to metastasis.2 The normal expression of Axin in esophageal carcinoma was obviously lower than that in atypical hyperplasia and normal mucosa, which indicated that the down-regulation expression of Axin could be related to carcinogenesis. The expression of Axin was correlated with fibromembranous invasion and lymph node metastasis,but not with cell differential degree, indicating Axin might be involved in infiltration and metastasis of esophageal carcinoma.3 The expression of MMP-14 in esophageal carcinoma was obviously higher than that in atypical hyperplasia and normal mucosa, which indicated that overexpression of MMP-14 could be involved in carcinogenesis. The expression of MMP-14 was correlated with cell differential degree, fibromembranous invasion and lymph node metastasis, which indicated MMP-14 might be involved in the development of esophageal carcinoma and contribute to infiltration and metastasis.4 There was a negative correlation between the expression ofβ-catenin and Axin protein, the same between Axin and MMP-14, but no relation betweenβ-catenin and MMP-14 in esophageal carcinoma tissue, which indicated down-regulation of Axin might attenuate the effect ofβ-catenin degraded by complex of Axin/APC/GSK-3β, and lead to the aberrant accumulation ofβ-catenin in cytoplasma. The low expression of Axin, directly or indirectly up-regulated the expression of MMP-14 through unknown factors, which promoted infiltration and metastasis of esophageal carcinoma. |
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