Preliminary Study On Anti-angiogenesis And Anti-tumor Activity Of Sinapine

This paper presented a comprehensive summary and discussion on the most important targets currently attracting a great deal of interest in contemporary anti-tumor drug design and discovery .This paper highlighted the literatures of sinapine, such as physical and chemical property, preparation methods, determination methods , bioactivities and toxicities,etc. This paper firstly elaborated the anti-angiogenesis of sinapine on the formation of microvessels and the anti-tumor effect of sinapine in S₁₈₀-bearing mice and its mechanism,which might provide useful information in finding better thoughts of exploitation from sinapine.Sinapine was purified by the procedure of pressurized-solvent extraction and microwave-assisted extraction from Semen Sinapis Albae.The contents of sinapine were determinated by ultra-violet spectrophpotometery(UV).The inhibition effects of sinapine on the chorioallantoic vessel were detected by the chick embryo chorioallantoic membrane model and discal-angiogenesis model. The mice models of implanted S₁₈₀ sarcoma were built up to observe the effect of sinapine on transplantation tumor's growth with intraperitoneal and orthotopic injection.The effect of sinapine on the micronuclei of polychromatic erythrocytes(MNPCEs) in mouse bone marrow cells induced by CTX was detected by micronucleus test of bone marrow PCE cell in mice.Changes in cell morphology in the S₁₈₀ solid tumor of mice were observed by normal Hematoxylin-eosin (HE) staining and acridine orange(AO) staining.DNA agarose electrophoresis was used to detect the characteristic changes of apoptosis in the effect of sinapine on the S₁₈₀ solid tumor of mice.The expressions of metastasis-associated gene Bcl-2 in the tumor tissue were determined using immunohistochemistry of PowerVision(non-biotin).The results indicated that sinapine could evidently prevent the formation of microvessels,resist the tumor growth in S₁₈₀-bearing mice,extend the life span of S₁₈₀A-bearing mice and a dose-response relationship existed. The results also showed that sinapine had an unconspicuous toxicity on the growth of mice,could decrease significantly the frequency of PCE micronucleus in mouse bone marrow cells induced by CTX, resist the expression of metastasis-associated gene Bcl-2 and promoted apoptosis of mouse S₁₈₀ sarcoma cells.