Effects Of All-Trans Retinoic Acid On The Human Ovarian Cancer HO8910 Cell Line

Objective: To investigate the effects of all-trans retinoic acid on the human ovarian cancer cell line HO8910.Methods: The human ovarian cancer cell line HO8910 cultured in vitro were treated with various concentrations of all-trans retinoic acid. The cell proliferation inhibition was detected by MTT assay; The morphologic changes of cell differentiation were observed under inverted microscope; The invasion capability was evaluated by transwell chambers; The expression level of adaptor protein Crk was measured by S-P immunocytochemical technique and Western-blot.Results: 1.ATRA of 10-7mol/l～10-5mol/l inhibited the proliferation of HO8910 cell significantly in a dose-dependent and time-dependent manner( P<0.05 ) and its IC50 was 2.331×10-5mol/l , 0.998×10-6mol/l ,0.891×10-7mol/l at 24hours,48hours and 72hours separately. 2.Treated by 10-6mol/l ATRA, HO8910 cells showed striking morphological characteristics of differentiation under microscope. 3.The number of invasive cells decreased significantly after treated by 10-6mol/l ATRA(P<0.05). 4.The result of S-P immunocytochemistry and Western-blot showed the expression of adaptor protein Crk was down-regulated obviously compared with the control group(P<0.05).Conclusion: ATRA can both inhibit the proliferation and invasion of human ovarian cancer cell line HO8910. These results may provide an effective and novel approach for clinical treatment of human ovarian cancer.