The Active Groups Activity Of Ti-O Films And Its Influence To Biocompatibility

In this work, Ti-O films with different crystal structure were synthesized by unbalanced magnetron sputtering system. Then the Ti-O films were treated by alkali solution, H₂SO₄ and H₂O₂ solution, and plasma immersion ion implantation (PIII) using ammonia gases to obtain functional groups (hydroxyl and amino). The crystal structures of films were characterized by the X-ray Diffraction (XRD).Fourier Transform Infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Scanning electron microscopy (SEM), contact-angle measurement and sheet resistance measurement were used to investigate the characteristics of Ti-O films and modified Ti-O film surface. Human umbilical vein endothelial cells (HUVEC) were in vitro seeded to the treated and untreated Ti-O films surface to evaluate the cell compatibility. The behavior of blood platelet adhesion were studied by light microscope and SEM after activation treatment to evaluate the blood compatibility. Furthermore, the relationship between the Ti-O film structure and its bioactivity is discussed in this work.The results revealed that films can produce functional groups activated by the way as mentioned above, and the groups firmly bonded to the surface. Compared to films of rutile, anatase film and film mixed with anatase and rutile possesses the better functional groups bioactivity. The results of SEM suggested that the surface morphology did not change after treated and the contact angle of the film surface decreased. The cell and platelet adhesion tests demonstrated that there were more cells and less platelet adhered to the treated Ti-O films surfaces than untreated ones. This result indicates the functional groups can promote the adhesion and growth of endothelial cells and reduce the number of adherent platelets. This paper thinks that obtaining basic hydroxyl on the premise of the acid hydroxyl existence on the surface of the Ti-0 films. In addition, the surface morphology and crystal structure also affect the films functional groups bioactivity.