Correlation Between The Expression Of Livin, Survivin And IGF-2 In HCC And The Clinical Significance

Objective:To study the expression of Livin and Survivin,the novel inhibitor of apoptosis protein(IAP)family members,in human hepatocellular carcinoma(HCC)and to explore the relationship between Livin,Survivin expression and parameters features,as well as the correlation of Livin,Survivin and IGF-2 in HCC.Methods:Expression of Livin,Survivin and IGF-2 in 60 HCC patients were assessed by Labelled Streptavindin biotin.Twelve candidate factors associated with survival were analyzed by the COX proportional hazards model,kaplan-Meier was used to estimate the survival rate.Results:1.Livin,Survivin and IGF-2 expression was detected in 32/60(53.3%), 39/60(65.0%),31/60(51.7%)respectively.The expression rate of Livin and Survivin was higher in HCC group than that of the adjacent non-tumorous liver and the normal liver groups(P＜0.05).2.There was no significant correlation between the expression of Livin and Survivin in HCC and the tumor size,capsule status,cirrhosis,the classification of Child-Paugh,TNM stage,except the portal vein tumor thrombus(P＜0.05).There was no significant correlation between the expression of IGF-2 and capsule status,the classification of Child-Paugh and cirrhosis.However,the positive rate of IGF-2 was correlated significantly with portal vein tumor thromb,liver cirrhosis and TNM stage (P＜0.05).There was a close correlation between the expression of Livin, Survivin and IGF-2(P＜0.01).3.The multivariative analysis determined Livin and Survivin were independent significant prognostic factors.Conclusion:1.Livin and Survivin are over-expressed in HCC tissues.The up regulation expression suggest that they participate in carcinogenesis.2.There was no correlation between the expression of Survivin and Livin in HCC.It indicated that there could be a different mechanism in the cells apoptosis.3.The positive expression of IGF-2 in HCC tissues was significantly higher than in normaltissues,which indicated that IGF-2 could be correlated with carcinogenesis.The up expression of Livin,Survivin and IGF-2 has a close correlation,holding a prognostic significancein HCC.4.The expression of Livin and Survivin was not correlated with clinicopathological features of the patients including the tumor size,capsule status,cirrhosis,the classification of Child-Paugh,TNM stage,except the portal vein tumor thrombus。The multivariative analysis determined Livin and Survivin were independent significant prognostic factors,it suggested that they are likely to be identified as a special index in diagnosis and a potential therapeutic target in HCC.