| The drug couple,which is the most foundational and simplest form in the prescription compatibility of traditional Chinese medicine,specially imply the prescription composed by two relative fixed Chinese medicine in clinic.It posseses the basic indication function of the complex prescription.The drug couple is the marrow of compatibility,and also the core of the prescription composed with drug.The study of drug couple is the foundation and the cut-point in the investigation of the prescription compatibility.Because it not just has the characteristics of complex prescription,but also the feature of simple to spread out modern research.So we can investigate the regularity of compatibility begin with the drug couple.Rhizoma Anemarrhenae and Cortex Phellodendri are frequntly used in the compatibility of Traditional Chinese medicine.The earliest appearance of them is in the Zishen Pellet from《Lan shi mi cang》written by Li Gao in Jing-dynasty.The drug couple composed by the Rhizoma Anemarrhenae and Cortex Phellodendri were used in lots of modern preparation.Because of the significance in studying the regularity of compatibility and the catholicity of the application with Rhizoma Anemarrhenae and Cortex Phellodendri,we choose the drug couple composed of Rhizoma Anemarrhenae and Cortex Phellodendri as the object to investigate,to establish foundation of the study on the modern preparation.This article on the foundation of concluding study progess on Rhizoma Anemarrhenae Cortex Phellodendri and drug couple,mainly deals with quality studies on Rhizoma Anemarrhenae and Cortex Phellodendri,studies on the extraction and purification process,studies on the chemical composition before and after compatibility,fingerprint.1.Quality studies:besides micro-identification and TLC qualitative test,moisture,total ash test,using UV-colorimetry and HPLC,using berberine hydrochloride,timosaponin AⅢand sarsasapogenin as reference components, determin the contents of berberine hvdrochloride,total saponins,total alkaloids and sarsasapogenin.2.Studies were carried on the extractionand purification process of total saponins and total alkaloids by reviewing the impact of ethanol concentration, ratio of herb to solvent,extraction time and times.Set up the L9(31)orthogonal test and confirmed the best extraction process as 8BV 70%ethanol distilling three times,1h per time.Extract of total saponins and total alkaloids was purified with macroeticular resins.Different kinds of resins were applied to review the static and dynamic adsorption ability,to optimize the concentration,volume and flow rate of eluant.It was confirmed that the best purification process was XDA-5 macroeticular resins,total saponins of eluant to dried resins being 1:8,eluted bv 7BY 80%ethanol after 7BV water with flow rate of 2ml/min.80%ethanol part was collected and dried under vacuum resulting in the total saponins content above 50.0%.It was confirmed that the best purification process was LX-18 macroeticular resins,total alkaloids of eluant to dried resins being 1:15,eluted by 7BV 50%ethanol after 7BV water with flow rate of 2ml/min.50%ethanol part was collected and dried under vacuum resulting in the total saponins content above 50.0%.It was confirmed that the best purification process was LX-18 macroeticular resins, total saponins and total alkaloids content above 50.0%.3.Studies on the chemical composition before and after compatibility: using UV-colorimetry and HPLC,using berberine hydrochloride and sarsasapogenin,timosaponin AⅢas reference components,to evaluate the influence to the component by the compatibility of the drug couple.4.Studies of HPLC fingerprint was conducted to ten origins of Rhizoma Anemarrhenae and Cortex Phellodendri,total saponins and total alkaloids purification.Their fingerprints were built up by optimizing chromatogram condition,to characterize common peaks separately,so that the quality of medicinal materials can be controlled.Referring to "Technical Specifications of Fingerprint Study of Chinese Traditional Medicine Injection (Interim Regulation)" decreed by SFDA and similarity evaluation system for chromatographic fingerprint of TCM(2004 A)set by China pharmacopoeia committee,the results showed the similarity of Rhizoma Anemarrhenae and Cortex Phellodendri,total saponins and total alkaloids purification were both from0.9～1.0.To evaluate the influence to the component by the compatibility of the drug couple by using drug couple fingerprint.
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